Photoacoustic and photothermal cytometry using photoswitchable proteins and nanoparticles with ultrasharp resonances
Photoacoustic and photothermal cytometry using photoswitchable proteins and nanoparticles with ultrasharp resonances
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DOI：
10.1002/jbio.201300140
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发表时间：
2015-01-01
影响因子：
2.8
通讯作者：
Zharov, Vladimir P.
中科院分区：
文献类型：
--
作者：
Galanzha, Ekaterina I.;Nedosekin, Dmitry A.;Zharov, Vladimir P.
光开关荧光蛋白（ppsps）在响应光的情况下具有可控的光谱位移，导致了细胞生物学的突破。然而，传统的光开关并不适用于弱荧光蛋白。作为一种替代方法，光热光谱学和光声光谱学在研究吸收非荧光蛋白和纳米颗粒方面显示出巨大的潜力。然而，在可切换的PT和PA探针的开发上取得的进展很少，这些探针的吸收光谱位移是可控的。在这里，我们介绍了光热可切换纳米颗粒（ptsn）的概念。为了证明这一概念，我们在体外实验中证明了癌细胞中传统磁性纳米颗粒团簇和金涂层磁性纳米颗粒团簇的快速、可逆磁PT开关，以及在体内分子靶向循环细胞的金纳米棒中非线性超尖等离子体共振的PT开关。我们发现，在静态条件下，具有相对缓慢开关的遗传编码psfp可以作为三模态荧光、PT和PA探针，而在动态流动条件下，具有超快开关的ptsn可以在近红外组织透明窗口中提供更高的PA灵敏度。本文还提出了非线性现象在超分辨光谱PT和PA细胞术、显微术以及超越衍射和光谱极限的光谱燃烧中的应用。
Photoswitchable fluorescent proteins (PSFPs) with controllable spectral shifts in emission in response to light have led to breakthroughs in cell biology. Conventional photoswitching, however, is not applicable to weakly fluorescent proteins. As an alternative, photothermal (PT) and photoacoustic (PA) spectroscopy have demonstrated a tremendous potential for studying absorbing nonfluorescent proteins and nanoparticles. However, little progress has been made in the development of switchable PT and PA probes with controllable spectral shifts in absorption. Here, we introduce the concept of photothermally switchable nanoparticles (PTSNs). To prove the concept, we demonstrated fast, reversible magnetic-PT switching of conventional and gold-coated magnetic nanoparticle clusters in cancer cells in vitro and PT switching of nonlinear ultrasharp plasmonic resonances in gold nanorods molecularly targeted to circulating cells in vivo. We showed that genetically encoded PSFPs with relatively slow switching can serve as triple-modal fluorescent, PT, and PA probes under static conditions, while PTSNs with ultrafast switching may provide higher PA sensitivity in the near-infrared window of tissue transparency under dynamic flow conditions. Application of nonlinear phenomena for super-resolution spectral PT and PA cytometry, microscopy, and spectral burning beyond the diffraction and spectral limits are also proposed.