Local RANKL gene transfer to the periodontal tissue accelerates orthodontic tooth movement
Local RANKL gene transfer to the periodontal tissue accelerates orthodontic tooth movement
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DOI：
10.1038/sj.gt.3302707
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发表时间：
2006-04-01
期刊：
影响因子：
5.1
通讯作者：
Mitani, H
中科院分区：
文献类型：
--
作者：
Kanzaki, H;Chiba, M;Mitani, H
据报道，不仅选择性牙槽骨吸收，而且核因子κ B配体受体激活剂（RANKL）的表达诱导的压缩侧的移动牙齿。许多报告已经描述了通过激活破骨细胞来药理学加速牙齿移动（TM）。然而，由于通过血液循环快速冲洗，需要每日全身给药或每日局部注射。以前，我们发现，每3天OPG基因转移到牙周组织抑制RANKL介导的破骨细胞生成和减少实验TM。因此，我们假设局部RANKL基因转移到牙周组织中会加速TM。用固定矫治丝将6周龄雄性Wistar大鼠的上颌第一磨牙向腭侧移动。在TM期间，将含有小鼠RANKL表达质粒的灭活日本血凝病毒（HVJ）包膜载体定期注射到上颌第一磨牙的腭部牙周组织中。局部RANKL基因转移显著增强牙周组织中RANKL表达和破骨细胞生成，而无任何全身效应。RANKL基因转移侧的TM率显著增加。总之，我们证明了RANKL基因转移到牙周组织激活破骨细胞生成和加速实验TM的量。局部RANKL基因转移可能是一种有用的工具，不仅可以缩短正畸治疗，而且可以移动牙齿与周围骨骼融合的强直牙齿。
It has been reported that not only selective alveolar-bone resorption, but also receptor activator of nuclear factor kappa B ligand (RANKL) expression is induced on the compressed side of an orthodontically moving tooth. Numerous reports have described the pharmacological acceleration of tooth movement (TM) through the activation of osteoclasts. However, because of rapid flush out by blood circulation, daily systemic administration or daily local injection is needed. Previously, we discovered that every-3-days OPG gene transfer to the periodontal-tissue inhibited RANKL-mediated osteoclastogenesis and diminished experimental TM. Therefore, we hypothesized that local RANKL gene transfer into the periodontal tissue would accelerate TM. The upper first molars of 6-week-old male Wistar rats were moved palatally using fixed orthodontic wires. The inactivated hemagglutinating-virus of Japan (HVJ) envelope vector containing the mouse RANKL expression plasmid was injected periodically into the palatal periodontal tissue of the upper first molars during TM. Local RANKL gene transfer significantly enhanced RANKL expression and osteoclastogenesis in periodontal tissue without any systemic effects. The TM rate was significantly increased in the RANKL gene transfer side. In conclusion, we demonstrated that transfer of the RANKL gene to the periodontal-tissue activated osteoclastogenesis and accelerated the amount of experimental TM. Local RANKL gene transfer might be a useful tool not only for shortening orthodontic treatment, but also for moving ankylosed teeth where teeth, fuse to the surrounding bone.