Two-step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombe
Two-step, extensive alterations in the transcriptome from G0 arrest to cell division in Schizosaccharomyces pombe
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DOI：
10.1111/j.1365-2443.2007.01079.x
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发表时间：
2007-05-01
期刊：
影响因子：
2.1
通讯作者：
Yanagida, Mitsuhiro
中科院分区：
文献类型：
--
作者：
Shimanuki, Mizuki;Chung, Soo-Yoel;Yanagida, Mitsuhiro
多细胞生物体中的体细胞处于G0状态，在该状态下细胞被停滞并最终分化。为了理解G0状态是如何维持的，必须鉴定在G0中特异性表达或抑制的基因，因为它们控制G0。在裂殖酵母裂殖酵母中，单倍体细胞在氮源饥饿下完全被抑制，具有高活力。我们检查了G0细胞和恢复营养生长过程中的细胞的整体转录组。大约20%的类似5000个基因的转录本在复制前发生的两步转换中增加或减少了四倍以上。前30丰富的成绩单在G0，23个被核糖体和分裂植物状态的转录相关的替代。八个确定的集群与不同的改变模式相似的2700转录本进行了注释的基因本体论。53个基因的破坏表明，其中9个是必要的，以支持适当的G0状态。这九个基因包括两个C2H2锌指转录因子，一个参与RNA聚合酶II磷酸化的细胞周期蛋白样蛋白，两个假定的自噬调节因子，一个G蛋白激活因子，和两个CBS结构域蛋白，可能参与AMP激活激酶。
Body cells in multicellular organisms are in the G0 state, in which cells are arrested and terminally differentiated. To understand how the G0 state is maintained, the genes that are specifically expressed or repressed in G0 must be identified, as they control G0. In the fission yeast Schizosaccharomyces pombe, haploid cells are completely arrested under nitrogen source starvation with high viability. We examined the global transcriptome of G0 cells and cells on the course to resume vegetative growth. Approximately 20% of the transcripts of similar to 5000 genes increased or decreased more than fourfold in the two-step transitions that occur prior to replication. Of the top 30 abundant transcripts in G0, 23 were replaced by ribosome- and translation-related transcripts in the dividing vegetative state. Eight identified clusters with distinct alteration patterns of similar to 2700 transcripts were annotated by Gene Ontology. Disruption of 53 genes indicated that nine of them were necessary to support the proper G0 state. These nine genes included two C2H2 zinc finger transcription factors, a cyclin-like protein implicated in phosphorylation of RNA polymerase II, two putative autophagy regulators, a G-protein activating factor, and two CBS domain proteins, possibly involved in AMP-activated kinase.