Cytologic characterization of pulmonary alveolar macrophages by enzyme histochemistry in plastic.
Cytologic characterization of pulmonary alveolar macrophages by enzyme histochemistry in plastic.
复制标题
通过塑料中的酶组织化学对肺泡巨噬细胞进行细胞学表征。
DOI：
10.1177/31.12.6631003
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
T. Pretlow
中科院分区：
文献类型：
--
作者：
A. Soufleris;T. P. Pretlow;A. Bartolucci;A. Pitts;A. Macfadyen;E. Boohaker;T. Pretlow
巨噬细胞的定量形态测量分析应有助于更精确地定义巨噬细胞在肿瘤性疾病以及淋巴和造血组织炎症性疾病中的作用。可用表型标记的有用性受到组织切片中巨噬细胞表达程度的影响。为了研究人体组织切片中巨噬细胞的表型标记，我们选择了肺，因为肺泡巨噬细胞在组织切片中比大多数其他组织中的巨噬细胞更容易在形态学上识别。在包埋在甲基丙烯酸酯中的新鲜固定的肺的 1-2 微米切片中，89.7% 的肺泡巨噬细胞具有组织化学上可证明的酸性磷酸酶； 86.2%，以萘酚ASD氯乙酸酯为底物的非特异性酯酶； 86.2%，以α-萘基丁酸酯为底物的非特异性酯酶； 80.5%，过氧化物酶； 75.8%是铁。就标记物的表达而言，观察到的肺泡巨噬细胞之间的异质性很有趣。这种异质性可能反映了它们被激活的程度、它们到达肺部的不同时间段以及它们当地环境的差异。除过氧化物酶外，当组织在液氮上储存后固定时，所有检查的标记物均与用新鲜组织进行固定时一样得到证实。酸性磷酸酶和非特异性酯酶与氯乙酸底物产生明亮的颜色，有利于形态分析。在液氮上储存组织对于用组织化学标记物和单克隆抗体表征巨噬细胞同样令人满意。
Quantitative morphometric analyses of macrophages should facilitate a more precise definition of the role of macrophages in neoplastic diseases and inflammatory diseases of lymphoid and hemopoietic tissues. The usefulness of the available phenotypic markers is affected by the extent to which they are expressed by macrophages in tissue sections. For our study of phenotypic markers for macrophages in human tissue sections, we selected lung, since pulmonary alveolar macrophages are more readily identified morphologically in tissue sections than macrophages in most other tissues. In 1-2 micron sections of freshly fixed lung embedded in methacrylate, 89.7% of pulmonary alveolar macrophages had histochemically demonstrable acid phosphatase; 86.2%, nonspecific esterase with naphthol ASD chloroacetate as the substrate; 86.2%, nonspecific esterase with alpha-naphthyl butyrate as the substrate; 80.5%, peroxidase; and 75.8%, iron. With respect to their expression of markers, the observed heterogeneity among pulmonary alveolar macrophages is interesting; this heterogeneity may reflect the degree to which they have been activated, the different periods of time since they arrived in the lung, and differences in their local environments. Except for peroxidase, all examined markers were as well demonstrated when tissues were fixed after storage over liquid nitrogen as when fixation was carried out with fresh tissue. Acid phosphatase and nonspecific esterase with the chloroactetate substrate gave bright colors that would facilitate morphometric analyses. The storage of tissue over liquid nitrogen will be equally satisfactory for the characterization of macrophages with histochemical markers and monoclonal antibodies.
病理人骨髓活检标本的酶组织化学和免疫组织化学。
影响因子：
20.3
作者：
Beckstead,JH;Halverson,PS;Ries,CA;Bainton,DF
通讯作者：
Bainton,DF