Adjuvanticity effect of sodium alginate on subcutaneously injected BCG in BALB/c mice
Adjuvanticity effect of sodium alginate on subcutaneously injected BCG in BALB/c mice
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DOI：
10.1016/j.micinf.2008.12.003
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发表时间：
2009-02-01
影响因子：
5.8
通讯作者：
Rafiee-Tehrani, Morteza
中科院分区：
文献类型：
--
作者：
Dobakhti, Faramarz;Naghibi, Taraneh;Rafiee-Tehrani, Morteza
卡介苗（BCG）是唯一可用的结核病疫苗。改良疫苗的研究目前是一个非常活跃的调查领域。本研究研究无菌海藻酸钠对BALB/c小鼠皮下接种卡介苗的佐剂作用。将卡介苗加海藻酸盐皮下接种小鼠，与单独接种相同途径的小鼠进行免疫应答和保护效果比较。与单独接种卡介苗组相比，皮下接种卡介苗加海藻酸盐组小鼠的增生性和延迟型超敏反应、ifn - γ、特异性抗分枝杆菌、总IgG、IgG1和IgG2a的产生均显著增加。在全身感染卡介苗后，与单独接种卡介苗的小鼠相比，接种卡介苗加海藻酸盐的小鼠的平均细菌计数更低。皮下注射卡介苗加海藻酸盐诱导的免疫应答明显优于标准卡介苗诱导的免疫应答。(C) 2008年由Elsevier Masson SAS出版。
Bacillus Calmette-Guerin (BCG) is the only available vaccine against tuberculosis. The research for an improved vaccine is currently a very active field of investigation. In the present study, adjuvanticity effect of sterile sodium alginate on subcutaneous BCG vaccination in BALB/c mice was investigated. Mice were vaccinated subcutaneously with BCG plus alginate and the immune response and protective effect were compared to those of mice vaccinated with BCG alone by the same route. Proliferative and delayed-type hypersensitivity responses, IFN-gamma, specific anti-mycobacterium, total IgG, IgG1 and IgG2a production were significantly higher in mice immunized subcutaneously with BCG plus alginate in comparison with results of mice immunized with BCG alone. Following systemic infection with BCG, mice vaccinated with BCG plus alginate had lower mean bacterial count compared to those vaccinated with BCG alone. The immune responses induced by subcutaneous administration of BCG plus alginate were significantly better than the responses induced by standard BCG vaccination. (C) 2008 Published by Elsevier Masson SAS.