A high-quality reference genome sequence ofSalvia miltiorrhizaprovides insights into tanshinone synthesis in its red rhizomes
A high-quality reference genome sequence ofSalvia miltiorrhizaprovides insights into tanshinone synthesis in its red rhizomes
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DOI：
10.1002/tpg2.20041
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发表时间：
2020-09-17
期刊：
影响因子：
4.2
通讯作者：
Li, Xingfeng
中科院分区：
文献类型：
--
作者：
Song, Zhenqiao;Lin, Caicai;Li, Xingfeng
丹参，又名丹参或丹参，是一种重要的传统中药，数千年来一直用于治疗心血管疾病和其他疾病。它也被认为是重要的中药模式植物。在这里，一个高质量的参考基因组OFS。丹参是通过结合PacBio长读测序和染色质相互作用图谱(Hi-C)技术产生的，导致染色体规模的组装一个594.75 Mb的基因组序列，重叠群N50为2.70Mb。这一组装展示了迄今为止产生的丹参基因组的最高水平的连续性。这就是。丹参基因组包含32,483个蛋白质编码基因，重复DNA含量约为64.84%。年轻LTRs的高比例提示最近INS发生了多个TE转位爆发。丹参。次生代谢途径特有的基因在TES中得到了扩增。丹参素。在红根韧皮部合成活性成分的韧皮部中发现了一个新的细胞色素P450基因簇。该参考基因组序列为进一步研究次生代谢合成途径和遗传改良奠定了基础。丹参。
Salvia miltiorrhizaBunge, also known as red sage or Danshen, is an important traditional Chinese medicine (TCM) that has been used for thousands of years to treat cardiovascular and other diseases. It is also considered an important model TCM plant. Here, a high-quality reference genome ofS. miltiorrhizawas generated by combining PacBio long-read sequencing and chromatin interaction mapping (Hi-C) technologies, resulting in the chromosome-scale assembly of a 594.75-Mb genome sequence with a contig N50 of 2.70 Mb. This assembly shows the highest level of continuity for a Danshen genome generated thus far. TheS. miltiorrhizagenome contained 32,483 protein-coding genes, with a repetitive DNA content of approximately 64.84%. The high percentage of young LTRs suggests that multiple TE transposition bursts occurred recently inS. miltiorrhiza. Genes unique to secondary metabolism pathways were expanded in theS. miltiorrhizagenome. A new CYP450 gene cluster was identified in the phloem of red roots where active components were synthesized. This reference genome sequence will facilitate future studies aimed at the elucidation of the secondary metabolism synthesis pathway and the genetic improvement ofS. miltiorrhiza.