Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus).: II.: Pathology
Experimental yellow fever virus infection in the golden hamster (Mesocricetus auratus).: II.: Pathology
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DOI：
10.1086/320200
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发表时间：
2001-05-15
影响因子：
6.4
通讯作者：
Tesh, RB
中科院分区：
文献类型：
--
作者：
Xiao, SY;Zhang, H;Tesh, RB
亚成年和成年仓鼠腹腔内接种10（6）TCID 50的黄热病（YF）病毒（Jimenez株）。每组4只动物每天接受组织学检查，持续9天。感染后第3天肝脏出现点状坏死，随后出现脂肪变性和局灶性融合坏死。在存活的仓鼠中，肝细胞再生在第8天开始，伴随着脂肪变性的减少。脾脏最初表现出淋巴增生，随后出现淋巴消耗和脾脏巨噬细胞的吞噬作用增加。在第5 - 7天观察到一过性局灶性胰腺腺泡坏死和点状肾上腺皮质坏死。免疫组化法检测肝、脾中病毒抗原。TUNEL分析显示肝细胞坏死呈动态变化，其活性与疾病的严重程度相对应。年轻（亚成年）动物的组织病理学变化更严重。YF仓鼠模型似乎是一个准确和廉价的实验系统，用于研究YF的病理生理学和治疗。
Subadult and adult hamsters were inoculated intraperitoneally with 10(6) TCID50 of yellow fever (YF) virus (Jimenez strain). Four animals from each group were subjected daily to histologic examination for 9 days. The liver showed spotty necrosis on day 3 after infection, which was followed by steatosis and focally confluent necrosis. In surviving hamsters, hepatocyte regeneration began on day 8, which was accompanied by decreasing steatosis. The spleen initially exhibited lymphoid hyperplasia, which was followed by lymphoid depletion and increased phagocytosis by splenic macrophages. Focal pancreatic acinar necrosis and spotty adrenal cortical necrosis were seen transiently between days 5 and 7. Viral antigen was detected immunohistochemically in the liver and the spleen. TUNEL analysis showed a dynamic change of hepatocyte necrapoptosis, with activity corresponding to the severity of disease. The histopathologic changes were more severe in younger (subadult) animals. The YF-hamster model appears to be an accurate and inexpensive experimental system for studying the pathophysiology and treatment of YF.