Buyang Huanwu Decoction can improve recovery of neurological function, reduce infarction volume, stimulate neural proliferation and modulate VEGF and Flk1 expressions in transient focal cerebral ischaemic rat brains
Buyang Huanwu Decoction can improve recovery of neurological function, reduce infarction volume, stimulate neural proliferation and modulate VEGF and Flk1 expressions in transient focal cerebral ischaemic rat brains
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DOI：
10.1016/j.jep.2007.06.007
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发表时间：
2007-09-05
影响因子：
5.4
通讯作者：
Shen, Jiangang
中科院分区：
文献类型：
--
作者：
Cai, Guangxian;Liu, Baiyan;Shen, Jiangang
补阳还五汤是中医治疗中风致残的经典方剂。为探讨其药理基础，我们观察了全方及其中草药成分对局灶性脑缺血大鼠神经行为表现和脑梗塞体积的影响。分别于大脑中动脉闭塞30min后第3、7、14天测定神经功能缺损评分和脑梗塞体积。结果表明，补阳还五汤及其中草药组分能明显改善脑缺血大鼠的神经行为表现，缩小脑梗塞体积。为了阐明其可能的治疗机制，我们通过检测胸腺嘧啶核苷类似物5-溴-2‘-脱氧尿苷(BrdU)的免疫组织化学染色来研究其对造血祖细胞的增殖作用，发现该方可刺激缺血脑内海马区和脑室下区(SVZ)的造血祖细胞的增殖。鉴于血管内皮生长因子(VEGF)及其受体胎肝激酶(Flk1)是重要的神经营养、神经保护和神经增殖因子，我们研究了血管内皮生长因子及其受体Flk1在脑缺血大鼠海马区、SVZ区和皮质的表达，发现该方能增加缺血脑SVZ和皮质中VEGF和Flk1阳性细胞的数量。结果表明，补阳还五汤对脑缺血大鼠神经功能恢复的作用与其促进神经前体细胞的增殖，增强脑内血管内皮生长因子和Flk的表达有关。(C)2007爱思唯尔爱尔兰有限公司。保留所有权利。
Buyang Huanwu Decoction is a classic formula for treating stroke-induced disability in traditional Chinese medicine (TCM). To explore its pharmacological basis, we investigated the effects of the whole formula and its herbal components on the neurological behavior performance and infarction volume in focal cerebral ischaemia rats. The neurological deficit scores and infarction volume were measured at days 3, 7 and 14 after 30 min of occlusion of middle cerebral artery. The results showed that Buyang Huanwu Decoction and its herbal components significantly improved the neurological behavior performances and reduced the infarction volume in the ischaemic brains. To elucidate the potential therapeutic mechanisms, we investigated the proliferation of progenitors by detecting the immunohistochemical staining of thymidine analog 5-bromo-2'-deoxyuridine (BrdU) and found that the formula stimulated the proliferation of the progenitors at hippocampus and subventricular zone (SVZ) in the ischaemic brains. As vascular endothelial growth factor (VEGF) and its receptor fetal liver kinase (Flk1) are important neurotrophic, neuroprotective and neuroproliferative factors, we studied the expressions of VEGF and Flk1 in the hippocampus, SVZ and cortex in the ischaemic brains and found that the formula led to increase the numbers of VEGF-positive and Flk1-positive cells in the SVZ and cortex in the ischaemic brains. The results indicate that the therapeutic effects of Buyang Huanwu Decoction for recovery of neurological deficits are associated with the stimulation of the proliferation of progenitors and the enhancement of the expressions of VEGF and Flk in ischaemic brains. (C) 2007 Elsevier Ireland Ltd. All rights reserved.