Abnormal galactosylation of immunoglobulin G in cerebrospinal fluid of multiple sclerosis patients
Abnormal galactosylation of immunoglobulin G in cerebrospinal fluid of multiple sclerosis patients
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DOI：
10.1177/1352458516631036
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发表时间：
2016-12-01
影响因子：
5.8
通讯作者：
Fassbender, Klaus
中科院分区：
文献类型：
--
作者：
Decker, Yann;Schomburg, Robert;Fassbender, Klaus
背景资料：糖基化改变已与发展的几种人类疾病和动物模型，包括多发性sclerosis.Objectives：我们的目的是要确定是否免疫球蛋白G半乳糖基化可能会改变多发性sclerosis.Methods：免疫球蛋白G是从血清和脑脊液中分离出的多发性硬化症或病毒性脑膜炎和对照组患者没有炎症或自身免疫性疾病的历史。一个凝集素为基础的测定被用来调查潜在的半乳糖基化修饰的免疫球蛋白G。结果和结论：从对照患者的脑脊液分离的免疫球蛋白G的半乳糖基化被发现是年龄和性别依赖性的。此外，免疫球蛋白G半乳糖基化显着改变脑脊液中，但在多发性硬化症患者的血清中。此外，这种改变与多发性硬化症的积极进展相关。最后，半乳糖基部分的损失并不简单地与炎症有关，因为在以脑炎症为特征的病毒性脑膜炎患者中没有检测到这种变化。
Background: Glycosylation alterations have been associated with the development of several human diseases and their animal models, including multiple sclerosis.Objectives: We aimed to determine whether immunoglobulin G galactosylation might be changed in multiple sclerosis.Methods: Immunoglobulin G was isolated from serum and cerebrospinal fluid of patients with multiple sclerosis or viral meningitis and control patients without history of inflammatory or autoimmune disease. A lectin-based assay was used to investigate potential galactosylation modifications of immunoglobulin G.Results and conclusion: Galactosylation of immunoglobulin G isolated from cerebrospinal fluid of control patients was found to be age- and gender-dependent. In addition, immunoglobulin G galactosylation was significantly altered in cerebrospinal fluid but not in serum of multiple sclerosis patients. Furthermore, this modification was correlated with an active progression of multiple sclerosis. Finally, the loss of galactosyl moieties was not simply associated with inflammation as no such change was detected in viral meningitis patients characterized by brain inflammation.