Transgenic hepatitis B: a new model of HBV infection.
Transgenic hepatitis B: a new model of HBV infection.
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DOI：
10.1038/s41598-017-02862-2
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发表时间：
2017-06-01
影响因子：
4.6
通讯作者：
Torbenson M
中科院分区：
文献类型：
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作者：
Daniel HD;Torbenson M
慢性B型肝炎（HBV）感染是世界范围内发病和死亡的主要原因。目前，对病毒进入细胞质和在细胞培养中持续复制的关键步骤所涉及的细胞蛋白和相关分子的了解有限。为了解决这些基本问题，我们设计并实施了一种新的B型肝炎模型：由完整病毒和外源基因组成的传染性转基因B型肝炎病毒。外源基因允许鉴定被转基因病毒感染的细胞。将转基因病毒用于功能测定以鉴定病毒复制所需的细胞蛋白。该测定重复鉴定了蛋白质UQCR10。在HepG 2和Huh 7细胞中恢复UQCR 10水平后，它们可以被转基因B肝炎病毒的完整病毒体感染。这些结果证明了这种新的转基因B型肝炎模型的有用性。
Chronic hepatitis B infection (HBV) is major cause of morbidity and mortality throughout the world. Currently there is limited understanding on the cellular proteins and related molecules involved in the critical steps of viral entry into the cytoplasm and persistent viral replication in cell culture. In order to address these fundamental questions, we designed and implemented a new model of hepatitis B: infectious transgenic hepatitis B virus composed of a complete virus plus a foreign gene. The foreign gene allows identification of cells that are infected by the transgenic virus. The transgenic virus was used in a functional assay to identify cellular proteins necessary for viral replication. This assay repeatedly identified the protein UQCR10. After restoring UQCR10 levels in HepG2 and Huh7 cells, they can be infected by intact virions of transgenic hepatitis B. These results demonstrate the usefulness of this new transgenic hepatitis B model.