Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)
Functional Characterization of Sex Pheromone Receptors in the Fall Armyworm (Spodoptera frugiperda)
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秋粘虫（草地贪夜蛾）性信息素受体的功能特征
DOI：
10.3390/insects11030193
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发表时间：
2020-03-01
期刊：
影响因子：
3
通讯作者：
Dong, Shuang-Lin
中科院分区：
文献类型：
--
作者：
Guo, Jin-Meng;Liu, Xiao-Long;Dong, Shuang-Lin
雄蛾触角上的信息素受体在识别雌蛾释放的性信息素中起着重要作用。草地贪夜蛾（Spodoptera frugiperda）是一种著名的外来入侵害虫，但其发生情况未见报道。本研究克隆了系统发育分析提示的6个候选PRs（SfruOR 6、11、13、16、56和62），并采用实时定量PCR（qPCR）方法分析了它们的组织性别表达谱。除SfruOR 6外，其余6个基因在触角中均高度特异表达，其中SfruOR 6、13和62为雄性特异性表达，而SfruOR 11、16和56为雄性偏好性表达，表明它们在性信息素感知中的作用。利用非洲爪蟾卵母细胞系统进行的功能分析进一步表明，SfruOR 13对主要的性信息素组分Z9-14：0Ac和信息素类似物Z9，E12-14：0Ac高度敏感，但对次要的信息素组分Z9-12：0Ac不太敏感; SfruOR 16对信息素组分Z9-14：0Ac反应较弱，但对信息素类似物Z9 - 14：0 H反应较强;其他四个候选PR对四种信息素组分和四种信息素类似物中的任何一种都不反应。本研究有助于阐明中国林蛙对信息素的感知，并为基于OR的害虫控制技术的开发提供潜在的基因靶点。
Pheromone receptors (PRs) found in the antennae of male moths play a vital role in the recognition of sex pheromones released by females. The fall armyworm (FAW), Spodoptera frugiperda, is a notorious invasive pest, but its PRs have not been reported. In this report, six candidate PRs (SfruOR6, 11, 13, 16, 56 and 62) suggested by phylogenetic analysis were cloned, and their tissue-sex expression profiles were determined by quantitative real-time PCR (qPCR). All six genes except for SfruOR6 were highly and specifically expressed in the antennae, with SfruOR6, 13 and 62 being male-specific, while the other three (SfruOR11, 16 and 56) were male biased, suggesting their roles in sex pheromone perception. A functional analysis by the Xenopus oocyte system further demonstrated that SfruOR13 was highly sensitive to the major sex pheromone component Z9-14:OAc and the pheromone analog Z9,E12-14:OAc, but less sensitive to the minor pheromone component Z9-12:OAc; SfruOR16 responded weakly to pheromone component Z9-14:OAc, but strongly to pheromone analog Z9-14:OH; the other four candidate PRs did not respond to any of the four pheromone components and four pheromone analogs. This study contributes to clarifying the pheromone perception in the FAW, and provides potential gene targets for developing OR-based pest control techniques.