Effects of regular exercise on sleep and activity status in aging and ClkRNAi Drosophila melanogaster
Effects of regular exercise on sleep and activity status in aging and ClkRNAi Drosophila melanogaster
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定期运动对衰老和 ClkRNAi 果蝇睡眠和活动状态的影响
DOI：
10.1080/09291016.2019.1566990
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发表时间：
2019
影响因子：
1.1
通讯作者：
Dan Cheng
中科院分区：
文献类型：
--
作者：
Qiufang Li;LanZheng;Fan Yang;Hanzhe Li;Jinxiu Li;Dan Cheng
核心时钟基因的完整表达是生物体内稳态调节的基础，Clk作为核心昼夜节律基因的驱动因子之一，在维持生物昼夜节律中起着至关重要的作用。在本研究中，我们使用GAL 4/UAS系统下调色素分散因子中Clk的表达，色素分散因子是一种由大脑中的小腹侧神经元（sLNvs）节律性释放的神经肽，并结合规律的运动。然后，我们使用果蝇活动监测系统收集活动并分析睡眠和活动状态。结果表明，基因正常表达非运动组6周龄果蝇的早晚活动高峰和昼夜总活动量均显著低于3周龄果蝇，白天无效睡眠增多。3周的ClkRNAi果蝇在双峰模式活动减弱方面表现出与6周正常果蝇相似的特征;虽然它们白天的睡眠质量较差，但6周的ClkRNAi果蝇表现出夜间睡眠量减少和睡眠潜伏期延长。虽然规律运动不能提高果蝇ClkRNAi的睡眠质量，但可以通过改善正常组与年龄相关的睡眠效率，显著提高果蝇白天的睡眠强度。
The intact expression of core clock genes is essential for organism homeostatic regulation andClock(Clk) as one of drivers in core circadian gene plays a crucial role to maintain circadian rhythm. In this study, we use GAL4/UAS system to downregulate the expression ofClkspecifically in pigment-dispersing factor, a neuropeptide rhythmically released from small ventral lateral neurons (sLNvs) in the brain and combined with regular exercise. Then, we collected the activities usingDrosophilaactivity monitor system and analyzed the sleep and activity status. The results showed that activity peaks in the morning and evening and total activities during the day and night of 6-week-old flies were significantly lower than those of 3-week-old flies in gene normal expression non-exercise group, and ineffective sleep increased during the day. The 3-weekClkRNAiDrosophilashowed similar characteristics with 6-week normal flies in the weakened bimodal pattern activities; while they have worse sleep quality during the day, the 6-weekClkRNAi flies exhibited a reduced nighttime sleep volume and prolonged sleep latency. Although the regular exercise could not enhance the sleep quality ofClkRNAiDrosophila, it can significantly improve daytime sleep intensity by ameliorating age-related sleep inefficiency in normal group.