Inconsistent association of Epstein-Barr virus with CD56 (NCAM)-positive angiocentric lymphoma occurring in sites other than the upper and lower respiratory tract
Inconsistent association of Epstein-Barr virus with CD56 (NCAM)-positive angiocentric lymphoma occurring in sites other than the upper and lower respiratory tract
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DOI：
10.1046/j.1365-2559.1996.278324.x
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发表时间：
1996-02-01
期刊：
影响因子：
6.4
通讯作者：
Suchi, T
中科院分区：
文献类型：
--
作者：
Kobashi, Y;Nakamura, S;Suchi, T
我们以前描述了9例血管中心性淋巴瘤的可能的自然杀伤（NK）细胞谱系与表面CD 3-CD 56+表型发生在网站以外的上，下呼吸道。本研究采用聚合酶链反应（PCR）检测EB病毒DNA（EBV-DNA），原位杂交（ISH）检测EB病毒编码的小RNA（EBERs），免疫组化检测石蜡切片中EB病毒确定的核抗原2（EBNA-2）和潜伏膜蛋白1（LMP-1），探讨EB病毒与淋巴瘤的关系。PCR和ISH产生了几乎相同的结果，EBER被确定在3例淋巴瘤细胞的细胞核中，其中2例在肿瘤细胞的细胞质中显示LMP-1而没有EBNA-2表达。分子遗传学分析显示，EB病毒被纳入这三个EBER阳性的情况下，无论是克隆或双克隆。通过重新评估其形态与每个病例的既定EBV状态，发现与EBV阳性肿瘤的相当可变和不规则的细胞组成相反，EBV阴性肿瘤由于其显著均匀的“胚样”外观而突出，并且可以被归类为母细胞性NK细胞淋巴瘤。EB病毒阳性病例与鼻NK细胞肿瘤的关系尚未明确。
We previously described nine cases of angiocentric lymphoma of a possible natural killer (NK)-cell lineage with a surface CD3- CD56+ phenotype occurring in sites other than the upper and lower respiratory tract. This study was performed to investigate the association of Epstein-Barr virus (EBV) with these lymphomas, using the polymerase chain reaction (PCR) for the presence of EBV-DNA, in situ hybridization (ISH) for EBV-encoded small RNAs (EBERs) and immunohistology for EBV-determined nuclear antigen-2 (EBNA-2) and latent membrane protein-1 (LMP-1) in paraffin sections. PCR and ISH produced almost identical results, and EBERs were identified in the nuclei of the lymphoma cells of three cases, two of which exhibited LMP-1 in the cytoplasm of tumour cells without EBNA-2 expression. Molecular genetic analysis revealed EBV to be incorporated into these three EBER-positive cases either clonally or biclonally. It was revealed by re-evaluation of their morphology with the established EBV status on each case that, in contrast to the rather variable and irregular cellular composition of the EBV-positive tumours, the EBV-negative tumours stood out because of their remarkably uniform 'blastoid' appearance, and could be grouped as blastic NK-cell lymphoma. The relationship of the EBV-positive cases with nasal NK-cell tumours has yet to be clarified.