Specific characteristic of radial glia in the human fetal telencephalon
Specific characteristic of radial glia in the human fetal telencephalon
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DOI：
10.1002/glia.20044
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发表时间：
2004-10-01
期刊：
影响因子：
6.2
通讯作者：
Zecevic, N
中科院分区：
文献类型：
--
作者：
Zecevic, N
采用电镜和免疫细胞化学方法，结合多种胶质细胞和神经元细胞标记物，分析了发育中的人脑端壁细胞的表型特征。结果表明，在胚胎发育过程中，新皮质增殖区出现了多种明确的细胞类型，并受到不同的调控。在妊娠5-6周，可以观察到三种主要的细胞类型。大多数增殖性心室区（VZ）细胞都有放射状胶质（RG）标记物，如vimentin、胶质纤维酸性蛋白（GFAP）和谷氨酸星形胶质细胞特异性转运蛋白（GLAST）抗体。这些RG细胞的一个亚群还表达神经元标志物β iii -微管蛋白、MAP-2和磷酸化的神经丝SMI-31，以及干细胞标志物巢蛋白，表明它们具有多电位能力。此外，仅与神经元标记物发生免疫反应的VZ细胞的存在表明出现了限制性神经元祖细胞。在3个月的人类皮质发生过程中，VZ内的多电位祖细胞数量逐渐减少，而限制性更强的祖细胞数量系统地增加。这些结果表明，在人端脑初始皮质发生过程中，多电位祖细胞与限制性神经元祖细胞和RG细胞共存。由于多电位VZ细胞在新皮层神经发生的主波中消失，RG和限制性神经元祖细胞似乎是皮层神经元的主要来源。因此，与啮齿动物相比，人类VZ和上复心室下区（SVZ）细胞的多样化开始得更早，也更明显。(C) 2004 Wiley-Liss, Inc。
Phenotypic characteristics of cells in the developing human telencephalic wall were analyzed using electron microscopy and immunocytochemistry with various glial and neuronal cell markers. The results suggest that multiple defined cell types emerge in the neocortical proliferative zones and are differentially regulated during embryonic development. At 5-6 weeks gestation, three major cell types are observed. Most proliferating ventricular zone (VZ) cells are labeled with radial glial (RG) markers such as vimentin, glial fibrillary acidic protein (GFAP), and glutamate astrocyte-specific transporter (GLAST) antibodies. A subpopulation of these RG cells also express the neuronal markers beta III-tubulin, MAP-2, and phosphorylated neurofilament SMI-31, in addition to the stem cell marker nestin, indicating their multipotential capacity. In addition, the presence of VZ cells that immunoreact only with neuronal markers indicates the emergence of restricted neuronal progenitors. The number of multipotential progenitors in the VZ gradually decreases, whereas the number of more restricted progenitors increases systematically during the 3-month course of human corticogenesis. These results suggest that multipotential progenitors coexist with restricted neuronal progenitors and RG cells during initial corticogenesis in the human telencephalon. Since the multipotential VZ cells disappear during the major wave of neocortical neurogenesis, the RG and restricted neuronal progenitors appear to serve as the main sources of cortical neurons. Thus, the diversification of cells in human VZ and overlying subventricular zone (SVZ) begins earlier and is more pronounced than in rodents. (C) 2004 Wiley-Liss, Inc.