Comprehensive profiling of lysine acetylproteome analysis reveals diverse functions of lysine acetylation in common wheat.
Comprehensive profiling of lysine acetylproteome analysis reveals diverse functions of lysine acetylation in common wheat.
复制标题
赖氨酸乙酰蛋白质组的综合分析揭示了普通小麦赖氨酸乙酰化的多种功能
DOI：
10.1038/srep21069
复制
发表时间：
2016-02-15
影响因子：
4.6
通讯作者：
Lin Q
中科院分区：
文献类型：
--
作者：
Zhang Y;Song L;Liang W;Mu P;Wang S;Lin Q
蛋白质的赖氨酸乙酰化是一种动态的、可逆的翻译后修饰，在真核生物和原核生物中都起着重要的调控作用。植物乙酰化蛋白质组的研究已经开展了很多。然而，到目前为止，还没有关于世界主要谷物作物普通小麦的数据。在这项研究中，我们进行了一个全球性的乙酰蛋白质组分析的普通小麦品种（小麦L.），中国的春天。总共在277个蛋白质上鉴定了416个赖氨酸修饰位点，这些位点参与了各种各样的生物过程。与以前的研究一致，很大比例的乙酰化蛋白质参与代谢过程。有趣的是，根据功能富集分析，26个乙酰化蛋白参与光合作用和卡尔文循环，表明赖氨酸乙酰化在这些过程中的重要作用。此外，蛋白质相互作用网络分析表明，不同的相互作用是由蛋白质乙酰化。这些数据代表了普通小麦中乙酰组的首次报道，并作为探索赖氨酸乙酰化在这种生物体中以及可能在所有植物中的生理作用的重要资源。
Lysine acetylation of proteins, a dynamic and reversible post-translational modification, plays a critical regulatory role in both eukaryotes and prokaryotes. Several researches have been carried out on acetylproteome in plants. However, until now, there have been no data on common wheat, the major cereal crop in the world. In this study, we performed a global acetylproteome analysis of common wheat variety (Triticum aestivum L.), Chinese Spring. In total, 416 lysine modification sites were identified on 277 proteins, which are involved in a wide variety of biological processes. Consistent with previous studies, a large proportion of the acetylated proteins are involved in metabolic process. Interestingly, according to the functional enrichment analysis, 26 acetylated proteins are involved in photosynthesis and Calvin cycle, suggesting an important role of lysine acetylation in these processes. Moreover, protein interaction network analysis reveals that diverse interactions are modulated by protein acetylation. These data represent the first report of acetylome in common wheat and serve as an important resource for exploring the physiological role of lysine acetylation in this organism and likely in all plants.