CONGENITAL ABSENCE OF CORTICOSPINAL TRACT DOES NOT SEVERELY AFFECT PLASTIC CHANGES OF THE DEVELOPING POSTNATAL SPINAL CORD
CONGENITAL ABSENCE OF CORTICOSPINAL TRACT DOES NOT SEVERELY AFFECT PLASTIC CHANGES OF THE DEVELOPING POSTNATAL SPINAL CORD
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DOI：
10.1016/j.neuroscience.2015.06.017
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发表时间：
2015-08-20
期刊：
影响因子：
3.3
通讯作者：
Zhou, L.
中科院分区：
文献类型：
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作者：
Huang, L.;Xian, Q.;Zhou, L.
皮质脊髓传入神经的到达和完善可能会影响脊髓和感觉运动网络的成熟。为了更好地理解这一点，我们研究了单突触肌肉传入的修订，活动相关基因，神经营养因子及其受体的表达在颈脊髓从出生后（P）0至21天。我们比较了对照组和Celsr 3垂直杆Emx 1小鼠，其中皮质脊髓轴突从未发展。皮质脊髓束（CST），标记的抗蛋白激酶C γ（PKC γ）抗体在背索，逐渐增加的控制，但从来没有可见的突变体。使用抗小白蛋白和胆碱乙酰转移酶双重免疫染色，本体感觉传入纤维和脊髓运动神经元之间的密切联系出现在P0，并逐渐消除之后，与对照组和突变小鼠之间没有差异。在这两种基因型中，小清蛋白阳性中间神经元的数量从P7到P21相似地增加，并且在P7观察到c-Jun蛋白的相当的上调。相反，对照样品中，睫状神经营养因子（CNTF）蛋白水平增加，从P0到P7和P14后逐渐下降，CNTF浓度是随时间变化的突变样品。在突变小鼠中，神经营养因子-3（NT 3）表达的动态分布也受到中度影响。在对照脊髓中，NT 3在P7时增加，在P14时减少，但在突变样品中保持更稳定。相反，脑源性神经营养因子（BDNF），原肌球蛋白相关激酶（Trk）B，TrkC，p75神经营养因子受体（p75（NTR））和胶质细胞源性神经营养因子（GDNF）的表达谱在两种基因型中相似。总之，与CNTF和NT 3表达的可能例外，大多数事件，伴随成熟的脊髓出现在很大程度上独立于皮质脊髓的输入。(C)2015年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The arrival and refinement of corticospinal afferents are likely to influence the maturation of the spinal cord and sensory-motor networks. To understand this better, we studied the revision of monosynaptic muscle afferents, the expression of activity-related genes, neurotrophins and their receptors in the cervical spinal cord from postnatal day (P) 0 to 21. We compared control and Celsr3 vertical bar Emx1 mice, in which corticospinal axons never develop. The corticospinal tract (CST), labeled by anti-protein kinase C gamma (PKC gamma) antibody in the dorsal funiculus, increased gradually in the control, but was never visible in the mutant. Using anti-parvalbumin and choline acetyltransferase double immunostaining, close contacts between proprioceptive afferent fibers and spinal motor neurons appeared at P0 and were gradually eliminated thereafter, with no difference between control and mutant mice. In both genotypes, the number of parvalbumin-positive interneurons increased similarly from P7 to P21, and a comparable upregulation of c-Jun protein was seen at P7. Contrary to control samples, in which ciliary neurotrophic factor (CNTF) protein levels increased from P0 to P7 and gradually decreased after P14, CNTF concentrations were time-invariant in mutant samples. The dynamic profile of neurotrophin-3 (NT3) expression was also moderately affected in mutant mice. In control spinal cord, NT3 was increased at P7 and decreased at P14, but remained more stable in mutant samples. In contrast, expression profiles of brain-derived neurotrophic factor (BDNF), tropomyosin-related kinase (Trk) B, TrkC, p75 neurotrophin receptor (p75(NTR)) and glial cellline-derived neurotrophic factor (GDNF) were similar in both genotypes. In conclusion, with the possible exception of CNTF and NT3 expression, most events that accompany maturation of the spinal cord appear largely independent of corticospinal inputs. (C) 2015 IBRO. Published by Elsevier Ltd. All rights reserved.