Determination of D-fructose in foodstuffs by an improved amperometric biosensor based on a solid binding matrix
Determination of D-fructose in foodstuffs by an improved amperometric biosensor based on a solid binding matrix
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基于固体结合基质的改进电流生物传感器测定食品中的 D-果糖
DOI：
10.1039/a900048h
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
S. Miertus
中科院分区：
文献类型：
--
作者：
M. Streďanský;A. Pizzariello;S. Stredanska;S. Miertus
基于固体结合基质 (SBM) 复合传感器的改进电流生物传感器已用于测定食品样品中的 D-果糖。 D-果糖脱氢酶 (EC 1.1.99.11) 被直接掺入包含 2-十六酮作为 SBM 和化学改性石墨的固体复合传感器中。使用六氰基铁酸盐(III)作为氧化还原介体，并通过电流分析法测量由D-果糖的存在引起的电流变化。描述了复合果糖生物传感器的电化学性质和特性。电流信号快速、可重复，并且与 50 × 10–6–10 × 10–3 mol l–1 范围内的 D-果糖浓度成线性比例，相关系数为 0.999。对 2 × 10-3 mol l-1D-果糖在 +0.20 V 与 SCE 下进行的一组测量得出稳态电流的相对标准偏差为 2.11%。通过温和氧化步骤使用化学改性石墨可以提高生物传感器对阴离子干扰物（如 L-抗坏血酸）的选择性。事实证明，该生物传感器可稳定保存 6 个月，并且该电极对 D-果糖的测定不受食品样品中常见的糖或其他干扰物的影响。该生物传感器用于部分食品样品中D-果糖的测定，结果与市售D-果糖酶光度试剂盒的结果一致。
An improved amperometric biosensor based on a solid binding matrix (SBM) composite transducer has been used for the determination of D-fructose in foodstuffs samples. The enzyme, D-fructose dehydrogenase (EC 1.1.99.11), was incorporated directly into a solid composite transducer containing both 2-hexadecanone as SBM and chemically modified graphite. Hexacyanoferrate(III) was used as a redox mediator and the current variation caused by the presence of D-fructose was measured amperometrically. The electrochemical properties and the characteristics of the composite fructose biosensors are described. The amperometric signals were fast, reproducible and linearly proportional to D-fructose concentrations in the range 50 × 10–6–10 × 10–3 mol l–1, with a correlation coefficient of 0.999. A set of measurements at +0.20 V versus SCE for 2 × 10–3 mol l–1D-fructose yielded a relative standard deviation for the steady-state current of 2.11%. The use of a chemically modified graphite by a mild oxidation step was shown to improve the biosensor selectivity against anionic interferents such as L-ascorbate. The biosensor proved to be stable for 6 months and the assay of D-fructose by this electrode was not influenced by the presence of sugars or other interferents commonly found in food samples. The biosensor was used for the determination of D-fructose in some food samples, and the results were consistent with those obtained with the commercially available D-fructose enzyme photometric kit.