The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds
The role of pannexin 1 hemichannels in ATP release and cell-cell communication in mouse taste buds
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DOI：
10.1073/pnas.0611280104
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发表时间：
2007-04-10
影响因子：
11.1
通讯作者：
Roper, Stephen D.
中科院分区：
文献类型：
--
作者：
Huang, Yi-Jen;Maruyama, Yutaka;Roper, Stephen D.
ATP已被证明是味蕾传入递质，但负责释放的细胞及其机制尚未确定。使用表达高亲和力神经递质受体的CHO细胞作为生物传感器，我们发现味觉刺激导致受体细胞通过小鼠味蕾中的泛连接蛋白1半通道分泌ATP。ATP进一步刺激其他味觉细胞释放第二种递质，血清素。这些结果提供了一种机制，连接细胞内Ca 2+释放味觉转导过程中分泌的传入递质，ATP，从受体细胞。它们还指示了味蕾内细胞间通信和信号处理的途径。
ATP has been shown to be a taste bud afferent transmitter, but the cells responsible for, and the mechanism of, its release have not been identified. Using CHO cells expressing high-affinity neurotransmitter receptors as biosensors, we show that gustatory stimuli cause receptor cells to secrete ATP through pannexin 1 hemichannels in mouse taste buds. ATP further stimulates other taste cells to release a second transmitter, serotonin. These results provide a mechanism to link intracellular Ca2+ release during taste transduction to secretion of afferent transmitter, ATP, from receptor cells. They also indicate a route for cell-cell communication and signal processing within the taste bud.