Membrane-mimetic films of asymmetric phosphatidylcholine lipid bolaamphiphiles.
Membrane-mimetic films of asymmetric phosphatidylcholine lipid bolaamphiphiles.
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DOI：
10.1021/la052125t
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发表时间：
2006-01
期刊：
影响因子：
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通讯作者：
Xue-Long Sun;Nilanjan Biswas;T. Kai;Z. Dai;R. Dluhy;E. Chaikof
中科院分区：
文献类型：
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作者：
Xue-Long Sun;Nilanjan Biswas;T. Kai;Z. Dai;R. Dluhy;E. Chaikof
跨越膜的磷脂双亲分子，无论是单独的还是作为多组分类脂膜的组成成分，都可能被证明是产生强健的生物活性膜模拟组装体的简单构建块。我们以前曾报道合成了不对称二烷基磷脂双亲化合物，它们在相反的链端含有酯连接的磷脂酰胆碱和胺的官能团。在这篇报告中，我们描述了通过末端胺与生物素偶联的磷脂双亲化合物的合成，该末端胺带有或不带有不同链长的聚氧乙烷间隔臂。用外荧光显微镜表征了生物素化双亲分子作为自组装单分子膜在空气-水界面的行为，发现连接体的类型和大小对结构域结构和pi-A等温线有很大影响。通过Langmuir-Blodgett沉积在涂有亲和素衍生聚电解质多层膜的平面基板上制备基片结合组件。值得注意的是，外反射红外光谱证实了在体外表现出延长稳定性的Bola两亲性薄膜的制备。
Membrane-spanning phospholipid bolaamphiphiles either alone or as a constituent of a multicomponent lipid membrane may prove to be facile building blocks for generating robust bioactive membrane-mimetic assemblies. We have previously reported the synthesis of asymmetric dialkyl phospholipid bolaamphiphiles that contain ester linked phosphatidylcholine and amine functionalities at opposite chain ends. In this report, we describe the synthesis of phospholipid bolaamphiphiles that are conjugated to biotin via the terminal amine with or without a poly(ethylene oxide) spacer arm of varying chain length. The behavior of biotinylated bolaamphiphiles as a self-assembled monolayer at an air-water interface was characterized by epi-fluorescence microscopy and revealed that domain structure and pi-A isotherms were substantially influenced by linker type and size. Substrate bound assemblies were produced by Langmuir-Blodgett deposition onto planar substrates coated with an avidin derivatized polyelectrolyte multilayer. Significantly, external reflectance infrared spectroscopy confirmed the fabrication of bolaamphiphile thin films that display extended stability in vitro.