The role of the silicatein-alpha interactor silintaphin-1 in biomimetic biomineralization.
The role of the silicatein-alpha interactor silintaphin-1 in biomimetic biomineralization.
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DOI：
10.1016/j.biomaterials.2008.12.021
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发表时间：
2009-03
期刊：
影响因子：
14
通讯作者：
M. Wiens;M. Bausen;F. Natalio;T. Link;U. Schlossmacher;W. Müller
中科院分区：
文献类型：
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作者：
M. Wiens;M. Bausen;F. Natalio;T. Link;U. Schlossmacher;W. Müller
海绵中的生物硅化是通过形成主要由硅酸盐组成的蛋白质丝来引发的。硅酸盐酶催化二氧化硅纳米球的缩合，产生对称的骨架元件（针状体）。为了在仿生方法中创建定制的生物二氧化硅结构，必须阐明对于细丝组装至关重要的蛋白质。 Silintaphin-1是模块化细丝的核心成分，也是针状体包裹层的一部分。它与其他蛋白质没有任何相似之处，除了存在对其作为支架/模板功能至关重要的相互作用结构域之外。在存在硅酸盐 silintaphin-1 的情况下，有利于体外丝的形成。此外，它还能将 γ-Fe2O3 纳米颗粒和表面固定的硅酸盐组装成棒状生物复合材料、合成针。因此，silintaphin-1 将有助于仿生方法，追求图案化生物硅基材料的受控形成。其与γ-Fe2O3纳米颗粒和固定化硅酸盐的结合将进一步激发未来的生物医学应用和临床诊断。
Biosilicification in sponges is initiated by formation of proteinaceous filaments, predominantly consisting of silicateins. Silicateins enzymatically catalyze condensation of silica nanospheres, resulting in symmetric skeletal elements (spicules). In order to create tailored biosilica structures in biomimetic approaches it is mandatory to elucidate proteins that are fundamental for the assembly of filaments. Silintaphin-1 is a core component of modularized filaments and also part of a spicule-enfolding layer. It bears no resemblance to other proteins, except for the presence of an interaction domain that is fundamental for its function as scaffold/template. In the presence of silicatein silintaphin-1 facilitates the formation of in vitro filaments. Also, it directs the assembly of γ-Fe2O3nanoparticles and surface-immobilized silicatein to rod-like biocomposites, synthetic spicules. Thus, silintaphin-1 will contribute to biomimetic approaches that pursue a controlled formation of patterned biosilica-based materials. Its combination with γ-Fe2O3nanoparticles and immobilized silicatein will furthermore inspire future biomedical applications and clinical diagnostics.