To what extent can portable fluorescence spectroscopy be used in the real-time assessment of microbial water quality?
To what extent can portable fluorescence spectroscopy be used in the real-time assessment of microbial water quality?
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DOI：
10.1016/j.scitotenv.2015.05.114
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发表时间：
2015-11-01
影响因子：
9.8
通讯作者：
Bridgeman, John
中科院分区：
文献类型：
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作者：
Baker, Andy;Cumberland, Susan A.;Bridgeman, John
在280 nm激发下，水体有机物在350 nm处发出的本征荧光与生化需氧量等水质参数密切相关。因此，在受污水影响的河流和地下水中，可以预期这些波长的荧光也将与微生物水质相关。在本文中，我们使用便携式荧光仪来评估该波长对的荧光强度与大肠杆菌计数在对比水质差的河流集水区之间的关系：在夸祖鲁-纳塔尔省，南非和西米德兰兹郡，英国。在所有集水区，我们证明了在非扰动（未过滤）样品上大肠杆菌计数的7对数范围内，荧光强度与大肠杆菌之间存在对数相关（r = 0.74）。在特定的集水区，荧光强度和大肠杆菌之间的关系是可变的，这表明了集水区特异性干扰的重要性。我们的研究证明了使用便携式荧光仪作为指示性微生物水质的初始筛选工具的潜力，并且非常适合于简单的污染情况，例如评估特定地点的河流或地下水中粪便污染的影响。(C) 2015年作者。Elsevier B.V.出版
The intrinsic fluorescence of aquatic organic matter emitted at 350 nm when excited at 280 nm correlates widely with water quality parameters such as biochemical oxygen demand. Hence, in sewage-impacted rivers and groundwater, it might be expected that fluorescence at these wavelengths will also correlate with the microbial water quality. In this paper we use a portable fluorimeter to assess the relationship between fluorescence intensity at this wavelength pair and Escherichia coli enumeration in contrasting river catchments of poor water quality: in KwaZulu-Natal, S. Africa and the West Midlands, UK. Across all catchments we demonstrate a log correlation (r = 0.74) between fluorescence intensity and E. coli over a seven-log range in E. coli enumerations on non-perturbed (unfiltered) samples. Within specific catchments, the relationship between fluorescence intensity and E. coli is more variable, demonstrating the importance of catchment-specific interference. Our research demonstrates the potential of using a portable fluorimeter as an initial screening tool for indicative microbial water quality, and one that is ideally suited to simple pollution scenarios such as assessing the impact of faecal contamination in river or groundwater at specific sites. (C) 2015 The Authors. Published by Elsevier B.V.