ATM-related Tel1 associates with double-strand breaks through an Xrs2-dependent mechanism
ATM-related Tel1 associates with double-strand breaks through an Xrs2-dependent mechanism
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DOI：
10.1101/gad.1099003
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发表时间：
2003-08-15
影响因子：
10.5
通讯作者：
Sugimoto, K
中科院分区：
文献类型：
--
作者：
Nakada, D;Matsumoto, K;Sugimoto, K
在芽殖酵母中，TEL 1编码与ATM密切相关的蛋白质。Xrs 2是Nbs 1的同系物，与Mre 11和Rad 50形成复合物。我们在这里表明，告诉协会与双链断裂（DSBs）通过依赖于Xrs 2的C末端的机制。尽管Xrs 2是DSB处DNA降解所必需的，但C-末端Xrs 2截短不影响降解。Tell和Xrs 2的C末端类似地参与DNA损伤后的细胞存活和Rad 53磷酸化。我们的研究结果表明，Tell与DNA损伤的关联是激活DNA损伤反应所必需的。
In budding yeast, TEL1 encodes a protein closely related to ATM. Xrs2 is an Nbs1 homolog and forms a complex with Mre11 and Rad50. We show here that Tell associates with double-strand breaks (DSBs) through a mechanism dependent on the C terminus of Xrs2. Although Xrs2 is required for the DNA degradation at DSBs, the C-terminal Xrs2 truncation does not affect the degradation. Tell and the C terminus of Xrs2 are similarly involved in cell survival and Rad53 phosphorylation after DNA damage. Our findings suggest that the Tell association with DNA lesions is required for the activation of DNA damage responses.