Tunable Mechanical, Antibacterial, and Cytocompatible Hydrogels Based on a Functionalized Dual Network of Metal Coordination Bonds and Covalent Crosslinking
Tunable Mechanical, Antibacterial, and Cytocompatible Hydrogels Based on a Functionalized Dual Network of Metal Coordination Bonds and Covalent Crosslinking
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基于金属配位键和共价交联功能化双网络的可调机械、抗菌和细胞相容性水凝胶
DOI：
10.1021/acsami.7b18821
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发表时间：
2018
影响因子：
9.5
通讯作者：
Ning Chengyun
中科院分区：
文献类型：
--
作者：
Yi Xin;He Jiapeng;Wang Xiaolan;Zhangg Yu;Tan Guoxin;Zhou Zhengnan;Chen Junqi;Chen Dafu;Wang Renxian;Tian Wei;Yu Peng;Zhou Lei;Ning Chengyun
由于再生医学需求的增加，组织工程成为一个快速发展的研究领域，而水凝胶因其三维结构而成为一种很有前途的组织工程支架。在本研究中，我们构建了一种新型的甲基丙烯酸明胶（GelMA）水凝胶（GelMA- his - zn (II)），这种由组氨酸和Zn2+修饰的水凝胶（GelMA- his - zn (II)），由于形成了共价交联和金属配位键的官能化双网络，具有出色的抗菌性能和可调的机械性能。金属配位键的引入不仅提高了GelMA共价交联水凝胶的强度，而且通过调整Zn2+的浓度使其机械性能可调。Zn2+和咪唑基团的协同作用赋予GelMA-His-Zn（II）水凝胶令人惊叹的抗菌性能（抑制率高达100%）。菌落形成单位的计数和压缩测试分别证实了GelMA-His-Zn（II）水凝胶的令人着迷的抗菌能力和可调的机械性能。此外，细胞计数试剂盒-8试验、细胞骨架染色试验和活/死试验证实了GelMA-His-Zn（II）水凝胶具有良好的细胞相容性。因此，GelMA-His-Zn（II）水凝胶在组织工程中具有广阔的应用前景。
Tissue engineering has become a rapidly developing field of research because of the increased demand from regenerative medicine, and hydrogels are a promising tissue engineering scaffold because of their three-dimensional structures. In this study, we constructed novel hydrogels of gelatin methacrylate (GelMA) hydrogels modified with histidine and Zn2+(GelMA-His-Zn(II)), which possessed fascinating antibacterial properties and tunable mechanical properties because of the formation of a functionalized dual network of covalent crosslinking and metal coordination bonds. The introduction of metal coordination bonds not only improves the strength of the GelMA hydrogels with covalent crosslinking but also makes their mechanical properties tunable via adjustments to the concentration of Zn2+. The synergistic effect of Zn2+and the imidazole groups gives the GelMA-His-Zn(II) hydrogels fascinating antibacterial properties (up to 100% inhibition). Counting the colony forming units and compression tests confirmed the fascinating antibacterial abilities and tunable mechanical properties, respectively, of the GelMA-His-Zn(II) hydrogels. In addition, Cell Counting Kit-8 assays, cytoskeletal staining assays, and live/dead assays confirmed the excellent cytocompatibility of the GelMA-His-Zn(II) hydrogels. Therefore, the GelMA-His-Zn(II) hydrogels are promising for applications in tissue engineering.