The microRNA476a‐RFL module regulates adventitious root formation through a mitochondria‐dependent pathway in Populus
The microRNA476a‐RFL module regulates adventitious root formation through a mitochondria‐dependent pathway in Populus
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microRNA476a-RFL 模块通过线粒体依赖性途径调节杨属不定根的形成
DOI：
10.1111/nph.17252
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发表时间：
2021
期刊：
影响因子：
9.4
通讯作者：
Keming Luo
中科院分区：
文献类型：
--
作者：
Changzheng Xu;Yuanxun Tao;Xiaokang Fu;Li Guo;Haitao Xing;Chaofeng Li;Ziwei Yang;Huili Su;Xianqiang Wang;Jian Hu;Di Fan;Vincent L. Chiang;Keming Luo
对于木本植物，无性繁殖效率在很大程度上取决于茎插穗基部不定根的形成。然而，尽管无性繁殖是目前树木育种和商业化最常见的做法，但我们对树木AR形态发生的分子机制的理解仍然有限。我们鉴定了杨树特异性miR 476 a作为创伤诱导的不定生根的调节因子，其通过协调线粒体稳态发挥作用。几个育性恢复因子样（RFL）基因编码的寡核苷酸定位的五肽重复蛋白。miR 476 a-RFL表达的遗传修饰揭示了miR 476 a/RFL介导的线粒体稳态的动态调节影响白杨AR的形成。通过外源性化学抑制剂对线粒体的干扰表明，miR 476 a/RFL介导的AR的形成依赖于线粒体的调控，而线粒体的调控又通过生长素信号途径起作用。因此，我们的研究结果建立了一个microRNA介导的AR发育所需的激素-生长素信号级联，为深入了解线粒体调控在植物发育可塑性中的作用提供了新的思路。
For woody plants, clonal propagation efficiency is largely determined by adventitious root (AR) formation at the bases of stem cuttings. However, our understanding of the molecular mechanisms contributing to AR morphogenesis in trees remains limited, despite the importance of vegetative propagation, currently the most common practice for tree breeding and commercialization.Here, we identifiedPopulus‐specific miR476a as a regulator of wound‐induced adventitious rooting that acts by orchestrating mitochondrial homeostasis.MiR476a exhibited inducible expression during AR formation and directly targeted severalRestorer of Fertility like(RFL) genes encoding mitochondrion‐localized pentatricopeptide repeat proteins. Genetic modification of miR476a‐RFLexpression revealed that miR476a/RFL‐mediated dynamic regulation of mitochondrial homeostasis influences AR formation in poplar. Mitochondrial perturbation via exogenous application of a chemical inhibitor indicated that miR476a/RFL‐directed AR formation depends on mitochondrial regulation that acts via auxin signaling.Our results thus establish a microRNA‐directed mitochondrion–auxin signaling cascade required for AR development, providing insights into the role of mitochondrial regulation in the developmental plasticity of plants.