IDENTIFICATION OF A TUMOR-SPECIFIC DETERMINANT ON NEOPLASTIC CELL SURFACES
IDENTIFICATION OF A TUMOR-SPECIFIC DETERMINANT ON NEOPLASTIC CELL SURFACES
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DOI：
10.1073/pnas.57.2.359
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发表时间：
1967-01-01
影响因子：
11.1
通讯作者：
GOLDBERG, AR
中科院分区：
文献类型：
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作者：
BURGER, MM;GOLDBERG, AR
对肿瘤细胞特异的凝集素进行纯化，发现是一种糖蛋白。流体动力学性质表明它是一种分子量约为26，000的不对称分子。凝集作用可迅速逆转，表明是半抗原样作用而不是酶作用。肿瘤特异性表面位点包括N-乙酰基-葡糖胺。该结论是基于N-乙酰基-葡糖胺、或壳二糖、或卵类粘蛋白、含有N-乙酰基-葡糖胺的化合物的独特半抗原抑制。据信唾液酸在肿瘤细胞表面上的含量高于正常细胞，它似乎也参与了凝集过程，尽管它可能不是特异性位点的一部分。
An agglutinin specific for neoplastic cells was purified and found to be a glycoprotein. The hydrodynamic properties suggest it to be an asymmetric molecule of about 26,000 molecular weight. Agglutination is rapidly reversible, indicating a haptenlike action rather than an enzymatic one. The tumor-specific surface site includes N-acetyl-glucosamine. This conclusion is based on a unique hapten inhibition by N-acetyl-glucosamine, or chitobiose, or ovomucoid, compounds which contain N-acetyl-glucosamine. Sialic acid, believed to occur in higher amounts on surfaces of tumor cells than on normal cells, seems also to be involved in the agglutination process although it is probably not part of the specific site.