Adenosine A1 receptor blockade mimics caffeine's attenuation of ethanol-induced motor incoordination
Adenosine A1 receptor blockade mimics caffeine's attenuation of ethanol-induced motor incoordination
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DOI：
10.1111/j.1742-7843.2004.pto950509.x
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发表时间：
2004-12-01
影响因子：
3.1
通讯作者：
Maginn, M
中科院分区：
文献类型：
--
作者：
Connole, L;Harkin, A;Maginn, M
评估咖啡因和乙醇的共同给药对大鼠在加速旋转杆（accelerod）上的运动协调的影响。乙醇（2.5 g/kg，口服）降低了加速器上的运动性能。咖啡因（5和20毫克/公斤，口服）的共同管理剂量依赖性衰减乙醇诱导的赤字。咖啡因（20 mg/kg，口服）单独不影响测试中的运动表现。由于咖啡因是一种非选择性腺苷受体拮抗剂，因此确定了腺苷A（1）和A（2A）受体阻滞剂减弱乙醇诱导的运动不协调的能力。预先给予腺苷A（1）受体拮抗剂DPCPX（5 mg/kg，腹腔注射）可减轻乙醇（2.5 g/ kg，口服）诱导的运动不协调。相比之下，预先给予腺苷A（2A）选择性拮抗剂SCH 58261（10 mg/kg腹腔注射）对乙醇诱导的运动缺陷无影响。这些数据表明，腺苷A（1）受体阻滞剂模拟咖啡因对乙醇诱导的运动失调的抑制作用，并可能有助于咖啡因抵消乙醇急性中毒作用的能力。
The effects of co-administration of caffeine and ethanol were assessed on the motor coordination of rats on the accelerating rotarod (accelerod). Ethanol (2.5 g/kg, orally) decreased motor performance on the accelerod. Co-administration of caffeine (5 and 20 mg/kg, orally) dose-dependently attenuated this ethanol-induced deficit. Caffeine (20 mg/kg, orally) alone did not affect motor performance in the test. As caffeine is a non-selective adenosine receptor antagonist the ability of adenosine A(1) and A(2A) receptor blockade to attenuate ethanol-induced motor incoordination was determined. Pre-treatment with the adenosine A(1) receptor antagonist DPCPX (5 mg/kg, intraperitoneally) attenuated ethanol (2.5 g/ kg, orally)-induced motor incoordination. By contrast, prior administration of the adenosine A(2A) selective antagonist SCH 58261 (10 mg/kg intraperitoneally) had no effect on the ethanol-induced motor deficit. These data demonstrate that adenosine A(1) receptor blockade mimics the inhibitory action of caffeine on ethanol-induced motor incorordination, and may contribute to the ability of caffeine to offset the acute intoxicating actions of ethanol.