Fluorescence-guided optical coherence tomography imaging for colon cancer screening: a preliminary mouse study.
Fluorescence-guided optical coherence tomography imaging for colon cancer screening: a preliminary mouse study.
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DOI：
10.1364/boe.3.000178
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发表时间：
2012-01-01
影响因子：
3.4
通讯作者：
Amiji M
中科院分区：
文献类型：
--
作者：
Iftimia N;Iyer AK;Hammer DX;Lue N;Mujat M;Pitman M;Ferguson RD;Amiji M
初步探讨了癌症筛查的新概念。一种装载了近红外(NIR)染料的癌症靶向剂局部应用于组织上，以突出癌症可疑位置并引导光学相干断层扫描(OCT)成像，该成像被用于进一步研究微米级的组织形态。在ApcMin小鼠身上进行的一项初步研究已经初步测试了这种新的癌症筛查方法。采用近红外染料标记、精氨酸-甘氨酸-天冬氨酸(RGD)多肽功能化的聚己内酯微球(PCLMPs)作为肿瘤靶向剂。该试剂识别ανβ3整合素受体(ABIR)，该受体由上皮性癌细胞过度表达。造影剂在小鼠结肠局部注射。孵育结束后，处死动物，用荧光引导的高分辨率光学相干断层扫描(OCT)成像观察结肠形态。初步结果显示，显微镜和激光诱导荧光成像均显示异常组织的优先染色，以及OCT对正常粘膜区域、早期不典型增生和腺癌的鉴别能力。虽然这项研究非常初步，但这项研究的结果表明，荧光引导的OCT成像可能是一种适合癌症筛查的方法。如果成功，这种方法可以被临床医生用来更可靠地在体内诊断早期癌症。
A new concept for cancer screening has been preliminarily investigated. A cancer targeting agent loaded with a near-infrared (NIR) dye was topically applied on the tissue to highlight cancer-suspect locations and guide optical coherence tomography (OCT) imaging, which was used to further investigate tissue morphology at the micron scale. A pilot study on ApcMin mice has been performed to preliminarily test this new cancer screening approach. As a cancer-targeting agent, poly(epsilon-caprolactone) microparticles (PCLMPs), labeled with a NIR dye and functionalized with an RGD (argenine-glycine-aspartic acid) peptide, were used. This agent recognizes the ανβ3 integrin receptor (ABIR), which is over-expressed by epithelial cancer cells. The contrast agent was administered topically in vivo in mouse colon. After incubation, the animals were sacrificed and fluorescence-guided high resolution optical coherence tomography (OCT) imaging was used to visualize colon morphology. The preliminary results show preferential staining of the abnormal tissue, as indicated by both microscopy and laser-induced fluorescence imaging, and OCT’s capability to differentiate between normal mucosal areas, early dysplasia, and adenocarcinoma. Although very preliminary, the results of this study suggest that fluorescence-guided OCT imaging might be a suitable approach for cancer screening. If successful, this approach could be used by clinicians to more reliably diagnose early stage cancers in vivo.