KINETIC-STUDIES OF FORMALDEHYDE BINDING IN TISSUE
KINETIC-STUDIES OF FORMALDEHYDE BINDING IN TISSUE
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DOI：
10.3109/10520299409106282
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发表时间：
1994-05-01
影响因子：
1.6
通讯作者：
HELANDER, KG
中科院分区：
文献类型：
--
作者：
HELANDER, KG
兔肝标本在缓冲的C-14-甲醛中固定不同时间至6天。固定约24小时后，同位素结合达到平台期；约100分钟后达到半最大结合水平。甲醛结合在37℃比25℃快，在pH 7.0比在pH 4.0快。在水中漂洗固定组织长达26天的过程中，同位素含量逐渐下降到预洗水平的10-20%，表明甲醛固定是一个可逆的过程。
Specimens of rabbit liver were fixed for various periods up to 6 days in buffered C-14-formaldehyde. Binding of the isotope reached a plateau after fixation for approximately 24 hr; the half-maximal binding level was reached after approximately 100 min. Formaldehyde binding at 37 C was faster than at 25 C, and faster at pH 7.0 than at pH 4.0. During rinsing of the fixed tissue in water for up to 26 days there was a progressive decrease in isotope content to 10-20% of the prerinse level, indicating that formaldehyde fixation is a reversible process.