The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis
The recombinant protein rSP03B is a valid antigen for screening dog exposure to Phlebotomus perniciosus across foci of canine leishmaniasis
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DOI：
10.1111/mve.12192
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发表时间：
2017-03-01
影响因子：
1.9
通讯作者：
Volf, P.
中科院分区：
文献类型：
--
作者：
Kostalova, T.;Lestinova, T.;Volf, P.
白蛉-宿主接触的频率可以通过针对白蛉唾液蛋白的宿主抗体水平来测量。重组唾液蛋白被认为是唾液腺匀浆（SGH）的有效替代品;然而，有必要证明这些抗原被针对同一物种的各种群体的抗体识别。白蛉（双翅目：Psychodidae）是欧洲西南部婴儿利什曼原虫（锥虫目：锥虫科）的主要媒介，广泛分布于葡萄牙至意大利。在本研究中，从来自遥远地区的自然暴露犬中采集血清样本，包括坎帕尼亚（意大利南部）、翁布里亚（意大利中部）和大都市里斯本地区（葡萄牙），其中P. perniciosus是唯一或主要的病媒种属。使用SGH和43-kDa黄色相关重组蛋白（rSP 03 B）筛选血清中的抗恶性疟原虫抗体。在所有区域中检测到识别SGH和rSP 03 B的抗体之间的强相关性，表明不同P. perniciosus种群之间存在大量抗原交叉反应性。在这一关系中，各区域之间没有发现显著差异。此外，rSP 03 B和天然黄色相关蛋白显示出共享相似的抗原表位，因为犬免疫球蛋白G（IgG）与天然蛋白的结合通过与重组形式预孵育而被抑制。这些结果表明，rSP 03 B应被视为白蛉暴露在整个地理分布的P. perniciosus的通用标记。
The frequency of sandfly-host contacts can be measured by host antibody levels against sandfly salivary proteins. Recombinant salivary proteins are suggested to represent a valid replacement for salivary gland homogenate (SGH); however, it is necessary to prove that such antigens are recognized by antibodies against various populations of the same species. Phlebotomus perniciosus (Diptera: Psychodidae) is the main vector of Leishmania infantum (Trypanosomatida: Trypanosomatidae) in southwest Europe and is widespread from Portugal to Italy. In this study, sera were sampled from naturally exposed dogs from distant regions, including Campania (southern Italy), Umbria (central Italy) and the metropolitan Lisbon region (Portugal), where P. perniciosus is the unique or principal vector species. Sera were screened for anti-P. perniciosus antibodies using SGH and 43-kDa yellow-related recombinant protein (rSP03B). Arobust correlation between antibodies recognizing SGH and rSP03B was detected in all regions, suggesting substantial antigenic cross-reactivity among different P. perniciosus populations. No significant differences in this relationship were detected between regions. Moreover, rSP03B and the native yellow-related protein were shown to share similar antigenic epitopes, as canine immunoglobulin G (IgG) binding to the native protein was inhibited by pre-incubation with the recombinant form. These findings suggest that rSP03B should be regarded as a universal marker of sandfly exposure throughout the geographical distribution of P. perniciosus.