INFLUENCE OF ARTERIAL DAMAGE AND WALL SHEAR RATE ON PLATELET DEPOSITION - EXVIVO STUDY IN A SWINE MODEL
INFLUENCE OF ARTERIAL DAMAGE AND WALL SHEAR RATE ON PLATELET DEPOSITION - EXVIVO STUDY IN A SWINE MODEL
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DOI：
10.1161/01.atv.6.3.312
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发表时间：
1986-05-01
期刊：
影响因子：
--
通讯作者：
FUSTER, V
中科院分区：
文献类型：
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作者：
BADIMON, L;BADIMON, JJ;FUSTER, V
为了研究血流对血小板与选定生物表面相互作用的影响，我们开发了一种体外灌注室系统。在本实验中，将去内皮化的猪主动脉和来自跟腱的I型胶原束暴露于天然或肝素化的猪血液中，时间为1至30分钟，流速对应于106至3380秒的壁剪切速率-1。在去内皮化血管壁上，血小板沉积随暴露时间和壁剪切速率的增加而增加，在灌注后5 ~ 10分钟达到最大值。在高剪切速率和长时间暴露（超过10分钟）下，血小板沉积从最大值下降，表明一些血小板被血流栓塞。超微结构分析显示血小板活化、扩散和脱颗粒。即使在长时间灌注（30分钟）和高壁剪切速率（3380秒-1）后，胶原诱导血小板在未达到饱和水平的情况下，以幂型曲线聚集。综上所述，暴露材料的反应性和局部剪切速率（由血流和通畅管腔截面定义）调节了血小板在损伤血管壁的沉积。
To study the influence of blood flow on platelet interaction with selected biological surfaces, we have developed an ex vivo perfusion chamber system. In the present experiments, deendothelialized pig aorta and collagen Type I bundles from Achilles tendon were exposed to either native or heparinized pig blood for periods of time ranging from 1 to 30 minutes, and for flow rates corresponding to wall shear rates of 106 to 3380 sec-1. On the deendothelialized vessel wall, platelet deposition increased with both exposure time and wall shear rate, reaching a maximum value between 5 and 10 minutes of perfusion. At high shear rates and long exposure time (over 10 minutes), platelet deposition decreased from maximum values, indicating that some platelets were embolized by the flow. Ultrastructure analysis of the specimens showed platelet activation, spreading, and degranulation. Collagen induced a progressive accumulation of platelets following a power type curve of aggregate growth with exposure time without reaching a saturation level, even after long perfusion times (30 minutes) and high wall shear rates (3380 sec-1). In conclusion, the reactivity of the exposed materials and the local shear rate, defined by the blood flow and the patent luminal cross section, regulate platelet deposition in injured vascular wall.