Data supporting characterization of CLIC1, CLIC4, CLIC5 and DmCLIC antibodies and localization of CLICs in endoplasmic reticulum of cardiomyocytes.
Data supporting characterization of CLIC1, CLIC4, CLIC5 and DmCLIC antibodies and localization of CLICs in endoplasmic reticulum of cardiomyocytes.
复制标题
DOI：
10.1016/j.dib.2016.03.061
复制
发表时间：
2016-06
期刊：
影响因子：
1.2
通讯作者：
Singh H
中科院分区：
文献类型：
--
作者：
Ponnalagu D;Rao SG;Farber J;Xin W;Hussain AT;Shah K;Tanda S;Berryman MA;Edwards JC;Singh H
氯离子细胞内通道 (CLIC) 蛋白彼此之间具有 60-70% 的序列同一性，并且专门定位于细胞内细胞器膜和细胞质。为了支持我们最近发表的论文“心脏线粒体氯离子胞内通道蛋白的分子特性”（Ponnalagu 等人，2016），表征不同 CLIC 旁系同源物/直系同源物（CLIC1、CLIC4、CLIC5 和 DmCLIC）抗体的特异性非常重要，这些抗体用于破译它们在心脏细胞中的定位。此外，还确定了 CLIC 在其他细胞器（如心肌细胞内质网 (ER)）中的定位。本文还提供了用于显示心脏组织中 CLIC 旁系同源物相对丰度的不同引物的数据以及所用各种 CLIC 抗体的特异性。我们证明心脏中的主要 CLIC，即 CLIC1、CLIC4 和 CLIC5，在内质网中表现出差异分布。 CLIC1 和 CLIC4 均显示出与内质网的共定位，而 CLIC5 则不然。
Chloride intracellular channel (CLICs) proteins show 60–70% sequence identity to each other, and exclusively localize to the intracellular organelle membranes and cytosol. In support of our recent publication, “Molecular identity of cardiac mitochondrial chloride intracellular channel proteins” (Ponnalagu et al., 2016), it was important to characterize the specificity of different CLIC paralogs/ortholog (CLIC1, CLIC4, CLIC5 and DmCLIC) antibodies used to decipher their localization in cardiac cells. In addition, localization of CLICs in the other organelles such as endoplasmic reticulum (ER) of cardiomyocytes was established. This article also provides data on the different primers used to show the relative abundance of CLIC paralogs in cardiac tissue and the specificity of the various CLIC antibodies used. We demonstrate that the predominant CLICs in the heart, namely CLIC1, CLIC4 and CLIC5, show differential distribution in endoplasmic reticulum. CLIC1 and CLIC4 both show co-localization to the endoplasmic reticulum whereas CLIC5 does not.