Diversity analysis of genes encoding Mfa1 fimbrial components in Porphyromonas gingivalis strains.
Diversity analysis of genes encoding Mfa1 fimbrial components in Porphyromonas gingivalis strains.
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DOI：
10.1371/journal.pone.0255111
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Hasegawa Y
中科院分区：
文献类型：
--
作者：
Sakae K;Nagano K;Furuhashi M;Hasegawa Y
牙龈卟啉单胞菌是一种革兰氏阴性厌氧细菌，与牙周病的发生有关。其菌株之间毒力因子（例如粘附菌毛）的遗传多样性影响细菌的致病性。牙龈卟啉单胞菌通常表达两种不同类型的菌毛：FimA 和 Mfa1。尽管编码主要 FimA 菌毛蛋白的 fimA 的遗传多样性已得到表征，但编码 Mfa1 菌毛成分（包括 Mfa1 至 Mfa5 蛋白）的基因尚未得到充分研究。因此，我们分析了 12 种未知牙龈卟啉单胞菌菌株和 62 种已知牙龈卟啉单胞菌菌株（总共 74 种）的基因型。 mfa1基因型主要分为两个基因型，53和70。此外，我们发现基因型70可以进一步分为两个亚型（70A和70B）。 mfa2 至 mfa4 的多样性与 mfa1 基因型一致，但没有观察到基因型 70 中的亚型。蛋白质结构模型显示 Mfa1 至 Mfa4 的基因型之间具有高度同源性。 mfa5基因被分为独立于其他基因型的五种基因型（A至E）。此外，基因型A进一步分为两个亚型（A1和A2）。令人惊讶的是，一些菌株具有两个mfa5基因，并且第二个mfa5仅出现在基因型E中。所有基因型中的Mfa5蛋白都显示出同源的C端一半，包括被IX型分泌系统识别的保守C端结构域。此外，仅在基因型 A 至 C 中检测到 N 端的冯维勒布兰德因子结构域。mfa1 基因型与 ragA 和 ragB 基因型（位于 mfa 基因簇的紧下游）部分相关，但与 fimA 基因型不相关。
Porphyromonas gingivalis, a gram-negative anaerobic bacterium, is associated with the development of periodontal disease. The genetic diversity in virulence factors, such as adhesive fimbriae, among its strains affects the bacterial pathogenicity. P. gingivalis generally expresses two distinct types of fimbriae, FimA and Mfa1. Although the genetic diversity of fimA, encoding the major FimA fimbrilin protein, has been characterized, the genes encoding the Mfa1 fimbrial components, including the Mfa1 to Mfa5 proteins, have not been fully studied. We, therefore, analyzed their genotypes in 12 uncharacterized and 62 known strains of P. gingivalis (74 strains in total). The mfa1 genotype was primarily classified into two genotypes, 53 and 70. Additionally, we found that genotype 70 could be further divided into two subtypes (70A and 70B). The diversity of mfa2 to mfa4 was consistent with the mfa1 genotype, although no subtype in genotype 70 was observed. Protein structure modeling showed high homology between the genotypes in Mfa1 to Mfa4. The mfa5 gene was classified into five genotypes (A to E) independent of other genotypes. Moreover, genotype A was further divided into two subtypes (A1 and A2). Surprisingly, some strains had two mfa5 genes, and the 2nd mfa5 exclusively occurred in genotype E. The Mfa5 protein in all genotypes showed a homologous C-terminal half, including the conserved C-terminal domain recognized by the type IX secretion system. Furthermore, the von Willebrand factor domain at the N-terminal was detected only in genotypes A to C. The mfa1 genotypes partially correlated with the ragA and ragB genotypes (located immediately downstream of the mfa gene cluster) but not with the fimA genotypes.
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Keystone-Pathogen假设。
DOI：
10.1038/nrmicro2873
发表时间：
2012-10
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
通讯作者：
--
影响因子：
28.3
作者：
Madej M;White JBR;Nowakowska Z;Rawson S;Scavenius C;Enghild JJ;Bereta GP;Pothula K;Kleinekathoefer U;Baslé A;Ranson NA;Potempa J;van den Berg B
通讯作者：
van den Berg B
影响因子：
3.7
作者：
Hutcherson JA;Bagaitkar J;Nagano K;Yoshimura F;Wang H;Scott DA
通讯作者：
Scott DA
影响因子：
7
作者：
Jackman, Shaun D.;Vandervalk, Benjamin P.;Birol, Inanc
通讯作者：
Birol, Inanc
影响因子：
2.8
作者：
Hasegawa, Yoshiaki;Iwami, Jun;Yoshimura, Fuminobu
通讯作者：
Yoshimura, Fuminobu