Response of cells on surface-induced nanopatterns: Fibroblasts and mesenchymal progenitor cells
Response of cells on surface-induced nanopatterns: Fibroblasts and mesenchymal progenitor cells
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DOI：
10.1021/bm0611533
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发表时间：
2007-05-01
影响因子：
6.2
通讯作者：
Hutmacher, Dietmar W.
中科院分区：
文献类型：
--
作者：
Khor, Hwei Ling;Kuan, Yujun;Hutmacher, Dietmar W.
用聚苯乙烯-嵌段-聚2-乙烯基吡啶两嵌段共聚物(PS-b-P2VP)和聚苯乙烯-嵌段-聚(4-乙烯基吡啶)两嵌段共聚物(PS-b-P4VP)的超薄膜在云母上形成了表面诱导纳米图案(SINPAT)。表面相互作用控制的微相分离导致在干式超薄膜表面形成化学非均相的表面纳米颗粒。利用两种不同的纳米颗粒表面，即蠕虫状和点状图案，研究了纳米范围内的形貌对细胞黏附、增殖和迁移的影响。原子力显微镜证实SINPAT在细胞培养条件下是稳定的。在纳米颗粒表面培养成纤维细胞和间充质祖细胞。相差显微镜和激光共聚焦显微镜显示成纤维细胞和间充质祖细胞更喜欢密集排列的蠕虫样图案。原子力显微镜显示，当细胞在两种截然不同的纳米颗粒上迁移时，它们对细胞外基质的重塑是不同的。
Ultrathin films of a poly(styrene)-block-poly(2-vinylpyrindine) diblock copolymer (PS-b-P2VP) and poly(styrene)-block-poly(4-vinylpyrindine) diblock copolymer (PS-b-P4VP) were used to form surface-induced nanopattern (SINPAT) on mica. Surface interaction controlled microphase separation led to the formation of chemically heterogeneous surface nanopatterns on dry ultrathin films. Two distinct nanopatterned surfaces, namely, wormlike and dotlike patterns, were used to investigate the influence of topography in the nanometer range on cell adhesion, proliferation, and migration. Atomic force microscopy was used to confirm that SINPAT was stable under cell culture conditions. Fibroblasts and mesenchymal progenitor cells were cultured on the nanopatterned surfaces. Phase contrast and confocal laser microscopy showed that fibroblasts and mesenchymal progenitor cells preferred the densely spaced wormlike patterns. Atomic force microscopy showed that the cells remodelled the extracellular matrix differently as they migrate over the two distinctly different nanopatterns.