Live-cell real-time imaging reveals role of mitochondria in cell-to-cell transmission of HIV-1
Live-cell real-time imaging reveals role of mitochondria in cell-to-cell transmission of HIV-1
复制标题
DOI：
10.1016/j.bbrc.2011.10.078
复制
发表时间：
2011-11-18
影响因子：
3.1
通讯作者：
Huang, Paul Lee
中科院分区：
文献类型：
--
作者：
Lee-Huang, Sylvia;Huang, Philip Lin;Huang, Paul Lee
我们使用活细胞，实时荧光成像的HIV-1感染的T细胞和未感染的靶细胞的共培养物，以检查线粒体在细胞间传播的病毒的行动。我们发现HIV感染细胞的线粒体进入未感染的靶细胞并促进病毒传播。我们表明，人类线粒体作为病毒的水库和载体，他们可以在细胞之间移动。我们的结果证实了这一点，即从HIV感染细胞中纯化的线粒体具有感染性，并且线粒体抑制剂阻断HIV传播。通过合胞体形成和HIV-1核心蛋白p24的产生来验证病毒在靶细胞中的感染和复制。我们的研究结果为病毒传播的细胞机制提供了新的见解，并将线粒体确定为病毒感染的新宿主靶点。(C)2011 Elsevier Inc. All rights reserved.
We used live-cell, real-time fluorescence imaging of co-cultures of HIV-1 infected T cells and uninfected target cells to examine the action of mitochondria during cell-to-cell transmission of the virus. We find that mitochondria of HIV infected cells enter uninfected target cells and advance viral spread. We show that human mitochondria serve as viral reservoirs and carriers and that they can move between cells. This was confirmed by our results that purified mitochondria from HIV infected cells are infectious, and that mitochondrial inhibitors block HIV transmission. Viral infection and replication in the target cells were verified by syncytial formation and HIV-1 core protein p24 production. Our results offer new insights into the cellular mechanisms of viral transmission and identify mitochondria as new host targets for viral infection. (C) 2011 Elsevier Inc. All rights reserved.