Trafficking and Subcellular Localization of Multiwalled Carbon Nanotubes in Plant Cells
Trafficking and Subcellular Localization of Multiwalled Carbon Nanotubes in Plant Cells
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DOI：
10.1021/nn102344t
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发表时间：
2011-01-01
期刊：
影响因子：
17.1
通讯作者：
Baba, Yoshinobu
中科院分区：
文献类型：
--
作者：
Serag, Maged F.;Kaji, Noritada;Baba, Yoshinobu
生物分子递送的主要障碍是穿过细胞膜并通过避免包埋到内体和其他裂解细胞器中来实现高细胞质浓度。受此目标的启发，我们研究了多壁碳纳米管（MWCNT）穿透植物原生质体（通过酶处理使植物细胞失去细胞壁）细胞膜的能力，并通过共聚焦成像和TEM技术研究了它们的内化机制。我们的结果确定了植物原生质体对多壁碳纳米管的内体逃逸摄取模式。此外，短 MWCNT（
Major barriers to delivery of biomolecules are crossing the cellular membranes and achieving a., high cytoplasmic concentration by circumventing entrapment into endosomes and other lytic organelles. Motivated by such aim, we have investigated the capability of multiwalled carbon nanotubes (MWCNTs) to penetrate the cell membrane of plant protoplasts (plant cells made devoid of their cell walls via enzymatic, treatment) and studied their internalization mechanism via confocal imaging and TEM techniques. Our results indentified an endosome-escaping uptake mode of MWCNTs by plant protoplasts. Moreover, short MWCNTs (