Anion size modulates salt taste in rats
Anion size modulates salt taste in rats
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DOI：
10.1152/jn.00621.2011
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发表时间：
2012-03-01
影响因子：
2.5
通讯作者：
Contreras, Robert J.
中科院分区：
文献类型：
--
作者：
Breza, Joseph M.;Contreras, Robert J.
首页--期刊主要分类--期刊细介绍--期刊详细文摘内容阴离子大小调节大鼠的盐味。神经生理学杂志107：1632-1648,2012。2011年12月28日首次出版；DOI：10.1152/jn.00621.2011。-本研究的目的是研究阴离子大小以及上皮钠通道和瞬时受体电位香草酸-1通道对大鼠鼓索(CT)神经元钠味觉反应的影响。我们记录了来自切断的CT神经的多单位反应和来自完整的、窄调和宽调的膝状神经节中盐敏感神经元的单细胞反应，同时记录了刺激诱发的总和电位，以在刺激接触舌上皮时发出信号。所有刺激物(0.3M氯化铵、0.5M蔗糖、0.03-0.5M氯化钠、0.01M柠檬酸、0.02M盐酸奎宁、0.1M氯化钾、0.03-0.5M葡萄糖酸钠)均以人工唾液作为冲洗和溶剂。我们使用药理拮抗剂苯扎米来评估ENaC介导的氯化钠反应，以及SB-366791和氯化十六烷基吡啶来评估TRPV1介导的反应。CT神经对氯化钠的反应在每个浓度下都大于葡萄糖酸钠；这主要归因于宽调谐的酸性通用型神经元对氯化钠的反应频率更高，潜伏期比葡萄糖酸钠更短。相比之下，狭窄调谐的氯化钠专家神经元对这两种盐的反应更相似，但在时间模式上有细微的差异。苯扎米仅降低了狭窄调谐神经元对氯化钠的CT神经和单细胞反应。令人惊讶的是，SB-366791和氯化十六烷基吡啶对CT神经或单细胞氯化钠反应没有影响。总体而言，我们的数据表明了真菌样乳头中的顶端ENaCs在外周味觉神经元处理有关钠的信息中发挥的关键作用；TRPV1通道的作用是一个谜。
Breza JM, Contreras RJ. Anion size modulates salt taste in rats. J Neurophysiol 107: 1632-1648, 2012. First published December 28, 2011; doi:10.1152/jn.00621.2011.-The purpose of this study was to investigate the influence of anion size and the contribution of the epithelial sodium channel (ENaC) and the transient receptor potential vanilloid-1 (TRPV1) channel on sodium-taste responses in rat chorda tympani (CT) neurons. We recorded multiunit responses from the severed CT nerve and single-cell responses from intact, narrowly tuned and broadly tuned, salt-sensitive neurons in the geniculate ganglion simultaneously with stimulus-evoked summated potentials to signal when the stimulus contacted the lingual epithelium. Artificial saliva served as the rinse and solvent for all stimuli (0.3 M NH4Cl, 0.5 M sucrose, 0.03-0.5 M NaCl, 0.01 M citric acid, 0.02 M quinine hydrochloride, 0.1 M KCl, and 0.03-0.5 M Na-gluconate). We used the pharmacological antagonist benzamil to assess NaCl responses mediated by ENaC, and SB-366791 and cetylpyridinium chloride to assess responses mediated by TRPV1. CT nerve responses were greater to NaCl than Na-gluconate at each concentration; this was attributed mostly to broadly tuned, acid-generalist neurons that responded with higher frequency and shorter latency to NaCl than Na-gluconate. In contrast, narrowly tuned NaCl-specialist neurons responded more similarly to the two salts, but with subtle differences in temporal pattern. Benzamil reduced CT nerve and single-cell responses only of narrowly tuned neurons to NaCl. Surprisingly, SB-366791 and cetylpyridinium chloride were without effect on CT nerve or single-cell NaCl responses. Collectively, our data demonstrate the critical role that apical ENaCs in fungiform papillae play in processing information about sodium by peripheral gustatory neurons; the role of TRPV1 channels is an enigma.