Mediastinal node and diaphragmatic targeting after intracavitary injection of avidin/99mTc-blue-biotin-liposome system
Mediastinal node and diaphragmatic targeting after intracavitary injection of avidin/99mTc-blue-biotin-liposome system
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DOI：
10.1002/jps.20516
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发表时间：
2006-01-01
影响因子：
3.8
通讯作者：
Goins, B
中科院分区：
文献类型：
--
作者：
Medina, LA;Calixto, SM;Goins, B
描述了一种用于将药物递送到纵隔淋巴结中的疾病扩展部位的方法。将亲和素/生物素-脂质体系统注入正常大鼠纵隔，观察其淋巴结分布。在腹膜内注射Tc-99 m-蓝-生物素-脂质体和胸膜内注射抗生物素蛋白后，注射剂量对脂质体淋巴靶向的影响，反之亦然。用闪烁成像法跟踪Tc-99 m-蓝-生物素-脂质体的运动，以确定药物动力学和器官摄取。注射Tc-99 m-蓝-生物素-脂质体后22 h进行组织生物分布研究。结果表明，与注射0.5mg剂量（2%-5%ID/器官，p < 0.05）相比，与注射每种药剂的腔体无关，5.0mg剂量的每种药剂导致更高的纵隔淋巴结靶向（8%-10%ID/器官）。当腹膜内注射Tc-99 m-蓝-生物素-脂质体和胸腔内注射亲和素时，观察到膈肌和相关药物的靶向作用。与此相反，胸膜，心包淋巴靶向观察时，Tc-99 m-蓝-生物素-脂质体注射在胸膜腔和抗生物素蛋白在腹膜。抗生物素蛋白/生物素-脂质体系统的腔内注射可潜在地用于递送可减少肿瘤转移和感染扩散到纵隔淋巴结的预防性药物。(c)2005 Wiley-Liss，Inc.
A method for delivering drugs to sites of disease extension in mediastinal nodes is described. Mediastinal node and lymphatic distributions were determined after intracavitary injection of the avidin/biotin-liposome system in normal rats. The effect of the injected dose on lymphatic targeting of liposomes after intraperitoneal injection of Tc-99m-blue-biotin-liposomes and intrapleural injection of avidin, and vice versa, is presented. Scintigraphic imaging was used to follow the movement of Tc-99m-blue-biotin-liposomes to determine the pharmacokinetics and organ uptake. Tissue biodistribution studies were performed 22 h after injection of the Tc-99m-blue-biotin-liposomes. Results indicated that independent of the cavity in which each agent was injected, a dose of 5.0 mg of each agent results in higher mediastinal node targeting (8%-10% ID/Organ) as compared with the injection of a 0.5 mg dose (2%-5% ID/Organ, p < 0.05). Targeting of diaphragm and associated lymphatics was observed when Tc-99m-blue-biotin-liposomes were injected in peritoneum and avidin in pleural space. In contrast, pleural, and pericardial lymphatic targeting was observed when Tc-99m-blue-biotin-liposomes were injected in pleural space and avidin in peritoneum. Intracavitary injection of the avidin/biotin-liposome system could potentially be used for the delivery of prophylactic drugs that could reduce tumor metastasis and infection spread to mediastinal nodes. (c) 2005 Wiley-Liss, Inc.