CHROMOSOME STRUCTURE IN PHAGE T4. I. CIRCULARITY OF THE LINKAGE MAP.
CHROMOSOME STRUCTURE IN PHAGE T4. I. CIRCULARITY OF THE LINKAGE MAP.
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噬菌体 T4 中的染色体结构。
DOI：
10.1073/pnas.51.5.775
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发表时间：
1964
影响因子：
11.1
通讯作者：
G. Denhardt
中科院分区：
文献类型：
--
作者：
G. Streisinger;R. Edgar;G. Denhardt
在提取时，噬菌体T4的每个颗粒产生一个DNA分子；3 .在遗传杂交中，所有的标记都被证明是相互联系的杂交还会产生短的杂合区域，这些杂合区域在基因组上随机分布杜尔曼和博纳认为，至少有一些杂合区与DNA链中的某种中断有关。另一方面，伯恩斯、托马斯和卡明斯对他们关于T4 DNA物理性质的实验结果的解释是，单链是连续的这个悖论可以通过对T4噬菌体的染色体做两个假设来解决。首先，它是一个具有末端冗余的线性分子；
On extraction, particles of phage T4 each yield a single DNA molecule;'-3 and in genetic crosses, all the markers prove to be linked to one another.4 Crossing also generates short heterozygous regions that are distributed randomly over the genome.5 Doermann and Boehner6 have suggested that at least some of the heterozygous regions involve interruptions of some sort in the DNA chain. Berns and Thomas7 and Cummings,8 on the other hand, interpret results of their experiments on the physical properties of T4 DNA to mean that the single strands are continuous.9 The paradox could be resolved by making two assumptions about the chromosome of phage T4. First, that it is a linear molecule with a terminal redundancy: