Considerations When Choosing High-Fat, High-Fructose, and High-Cholesterol Diets to Induce Experimental Nonalcoholic Fatty Liver Disease in Laboratory Animal Models.
Considerations When Choosing High-Fat, High-Fructose, and High-Cholesterol Diets to Induce Experimental Nonalcoholic Fatty Liver Disease in Laboratory Animal Models.
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DOI：
10.1093/cdn/nzab138
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发表时间：
2021-12
影响因子：
4.8
通讯作者：
Pellizzon MA
中科院分区：
文献类型：
--
作者：
Radhakrishnan S;Yeung SF;Ke JY;Antunes MM;Pellizzon MA
非酒精性脂肪性肝病（NAFLD）与代谢性疾病（包括肥胖、葡萄糖耐受不良和胰岛素抵抗）错综复杂地相关，并且包括一系列疾病，包括脂肪变性、非酒精性脂肪性肝炎（NASH）和纤维化。消耗高脂肪（HF;约40 kcal%脂肪，包括含有较高浓度饱和脂肪和反式脂肪的脂肪）、高果糖（HFr）和高胆固醇（HC）饮食的啮齿动物显示出NASH的许多临床相关特征，沿着其他代谢紊乱。C57 BL/6小鼠是最常用的动物模型，因为它们在数月的喂养后可发生显著的代谢紊乱，包括严重的NASH和纤维化，但其他模型也是易感的。含有这些不同因素的大量饮食（即，HF、HFr和HC）单独或联合使用，使饮食的选择变得困难。本方法学综述描述了这些营养素操作对小鼠、大鼠、豚鼠、仓鼠和非人灵长类动物NAFLD表型的疗效。本文提供了高脂肪、果糖和胆固醇饮食如何影响小鼠、大鼠、仓鼠、豚鼠和非人灵长类动物的非酒精性脂肪性肝炎和代谢性疾病的例子。
Nonalcoholic fatty liver disease (NAFLD) is intricately linked to metabolic disease (including obesity, glucose intolerance, and insulin resistance) and encompasses a spectrum of disorders including steatosis, nonalcoholic steatohepatitis (NASH), and fibrosis. Rodents consuming high-fat (HF; ∼40 kcal% fat including fats containing higher concentrations of saturated and trans fats), high-fructose (HFr), and high-cholesterol (HC) diets display many clinically relevant characteristics of NASH, along with other metabolic disorders. C57BL/6 mice are the most commonly used animal model because they can develop significant metabolic disorders including severe NASH with fibrosis after months of feeding, but other models also are susceptible. The significant number of diets that contain these different factors (i.e., HF, HFr, and HC), either alone or in combination, makes the choice of diet difficult. This methodology review describes the efficacy of these nutrient manipulations on the NAFLD phenotype in mice, rats, guinea pigs, hamsters, and nonhuman primates. This article provides examples of how diets high in fat, fructose, and cholesterol influence nonalcoholic steatohepatitis and metabolic disease in mice, rats, hamsters, guinea pigs, and nonhuman primates.