Large-scale monitoring of host cell gene expression during HIV-1 infection using cDNA microarrays
Large-scale monitoring of host cell gene expression during HIV-1 infection using cDNA microarrays
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DOI：
10.1006/viro.1999.0044
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发表时间：
2000-01-05
期刊：
影响因子：
3.7
通讯作者：
Katze, MG
中科院分区：
文献类型：
--
作者：
Geiss, GK;Bumgarner, RE;Katze, MG
人类免疫缺陷病毒1型（HIV-1）感染在mRNA和蛋白质水平上改变宿主细胞基因的表达。为了在mRNA水平上更全面地了解HIV感染对CD 4阳性T细胞的整体影响，我们在感染后2天和3天（p.i.）HIV-1在感染后2天，宿主细胞基因表达变化不大，但在感染后3天，20个细胞基因被鉴定为差异表达。参与T细胞信号传导、亚细胞运输和转录调控的基因，以及几个未表征的基因，都是其mRNA受到差异调控的基因。这些结果支持了HIV-1感染改变了广泛的细胞基因表达的假设，并为未来差异表达mRNA产物的功能研究提供了一个框架。(C)北京大学出版社.
Human immunodeficiency virus type 1 (HIV-1) infection alters the expression of host cell genes at both the mRNA and protein levels. To obtain a more comprehensive view of the global effects of HIV infection of CD4-positive T-cells at the mRNA level, we performed cDNA microarray analysis on approximately 1500 cellular cDNAs at 2 and 3 days postinfection (p.i.) with HIV-1. Host cell gene expression changed little at 2 days p.i,, but at 3 days p.i. 20 cellular genes were identified as differentially expressed. Genes involved in T-cell signaling, subcellular trafficking, and transcriptional regulation, as well as several uncharacterized genes, were among those whose mRNAs were differentially regulated. These results support the hypothesis that HIV-1 infection alters expression of a broad array of cellular genes and provides a framework for future functional studies on the differentially expressed mRNA products. (C) 2000 Academic Press.