Arthropod- and host-specific Borrelia burgdorferi bbk32 expression and the inhibition of spirochete transmission
Arthropod- and host-specific Borrelia burgdorferi bbk32 expression and the inhibition of spirochete transmission
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DOI：
10.4049/jimmunol.164.10.5344
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发表时间：
2000-05-15
影响因子：
4.4
通讯作者：
Flavell, RA
中科院分区：
文献类型：
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作者：
Fikrig, E;Feng, W;Flavell, RA
抗BBK 32（伯氏疏螺旋体纤连蛋白结合蛋白）和BBK 50（感染期间合成的两种抗原）的抗血清可保护小鼠免受实验性莱姆病传播的莱姆病。因此，B。研究了肩突硬蜱和鼠宿主内的burgdorferi bbk 32和bbk 50表达，以及BBK 32和BBK 50抗血清在整个载体-宿主生命周期中对螺旋体的影响。bbk 32和bbk 50的mRNA和蛋白首先在饱食的蜱中被检测到，证明在载体内的调节表达，然后bbk 32在小鼠皮肤接种部位的表达增加。在播散性鼠感染期间，bbk 32和bbk 50在几种鼠组织中表达，并且在30天时心脏和脾脏中的mRNA水平最高。BBK 32抗血清保护小鼠免受蜱传B、伯氏螺旋体感染，并且在饱食免疫小鼠的蜱虫内螺旋体数量减少90%。此外，75%的蜱虫在蜕皮时没有保留螺旋体，随后B，成年蜱传播的伯氏疏螺旋体受损。B. burgdorferi B的幼虫被I.这些数据表明bbk 32和bbk 50在蜱虫充血期间表达，并且BBK 32抗血清可以在载体-宿主生命周期的各个阶段干扰螺旋体传播。这些研究提供了对莱姆疏螺旋体病和其他媒介传播疾病的免疫机制的见解。
Antisera to BBK32 (a Borrelia burgdorferi fibronectin-binding protein) and BBK50, two Ags synthesized during infection, protect mice from experimental syringe-borne Lyme borreliosis. Therefore, B. burgdorferi bbk32 and bbk50 expression within Ixodes scapularis ticks and the murine host, and the effect of BBK32 and BBK50 antisera on spirochetes throughout the vector-host life cycle were investigated. bbk32 and bbk50 mRNA and protein were first detected within engorged ticks, demonstrating regulated expression within the vector, Then bbk32 expression increased in mice at the cutaneous site of inoculation. During disseminated murine infection, bbk32 and bbk50 were expressed in several murine tissues, and mRNA levels were greatest in the heart and spleen at 30 days. BBK32 antisera protected mice from tick-borne B, burgdorferi infection and spirochete numbers were reduced by 90% within nymphs that engorged on immunized mice. Moreover, 75% of these ticks did not retain spirochetes upon molting, and subsequent B, burgdorferi transmission by adult ticks was impaired. Larval acquisition of B, burgdorferi by I. scapularis was also inhibited by BBK32 antisera, These data demonstrate that bbk32 and bbk50 are expressed during tick engorgement and that BBK32 antisera can interfere with spirochete transmission at various stages of the vector-host life cycle. These studies provide insight into mechanisms of immunity to Lyme borreliosis and other vector-borne diseases.