MITOTIC REGULATOR PROTEIN RCC1 IS COMPLEXED WITH A NUCLEAR RAS-RELATED POLYPEPTIDE
MITOTIC REGULATOR PROTEIN RCC1 IS COMPLEXED WITH A NUCLEAR RAS-RELATED POLYPEPTIDE
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DOI：
10.1073/pnas.88.23.10830
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发表时间：
1991-12-01
影响因子：
11.1
通讯作者：
PONSTINGL, H
中科院分区：
文献类型：
--
作者：
BISCHOFF, FR;PONSTINGL, H
我们曾报道从人染色质中分离纯化出一个由两个蛋白质组成的复合体，它由一个47 kDa的组分RCC1和一个25 kDa的蛋白质组成。RCC1是有丝分裂的负调控因子。在这里，我们显示了25 kDa的蛋白具有与ras相关的序列。它与鸟嘌呤核苷酸结合，过量的镁离子和GDP或GTP使该复合体解离。免疫荧光研究和生化性质表明，与RAS家族的大多数成员不同，这种多肽以可溶单体的形式存在于核质中，是络合形式的25倍。我们将这个多肽命名为RAN，即ras相关核蛋白。
We previously reported the purification of a complex of two proteins from human chromatin, consisting of a 47-kDa component called RCC1, which is a negative regulator of mitosis, and a 25-kDa protein. Here we show that the 25-kDa protein has a ras-related sequence. It binds guanine nucleotides, and excess Mg2+ and GDP or GTP dissociate the complex. Immunofluorescence studies and biochemical properties indicate that this polypeptide, in contrast to most members of the Ras family, is present in the nucleoplasm as a soluble monomer, in 25-fold excess over the complexed form. We designate this polypeptide Ran, for ras-related nuclear protein.