Cytochrome P450 complement (CYPome) of the avermectin-producer Streptomyces avermitilis and comparison to that of Streptomyces coelicolor A3(2)
Cytochrome P450 complement (CYPome) of the avermectin-producer Streptomyces avermitilis and comparison to that of Streptomyces coelicolor A3(2)
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DOI：
10.1016/s0006-291x(03)01231-2
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发表时间：
2003-08-01
影响因子：
3.1
通讯作者：
Kelly, SL
中科院分区：
文献类型：
--
作者：
Lamb, DC;Ikeda, H;Kelly, SL
链霉菌属产生约三分之二的天然抗生素和广泛的其他次级代谢产物，包括抗蠕虫剂，抗肿瘤剂，抗真菌剂和除草剂。新完成的阿维菌素产生菌阿维菌素链霉菌的基因组序列包含33个细胞色素P450（CYP），比天蓝色链霉菌A3中观察到的18个多得多（2）。一些可能的代谢功能与它们的基因组定位和生物信息学分析一起报道。发现了7个全新的类属，以及在S. coelicolor A3（2）.发现了与保守子相关的不寻常的CYP 157形式的存在，其中CYP 157形式在两种S。阿维菌素和S. coelicolor A3（2）偏离了先前公认的CYP K-螺旋内EXXR基序的规则。在这一范围的CYP中，有与阿维菌素、菲律宾菌素、土臭素和戊烯内酯生物合成以及未知的次级代谢途径相关的形式。(C)2003年爱思唯尔公司All rights reserved.
The genus Streptomyces produces about two-thirds of naturally occurring antibiotics and a wide array of other secondary metabolites, including antihelminthic agents, antitumor agents, antifungal agents, and herbicides. The newly completed genome sequence of the avermectin-producing bacterium Streptomyces avermitilis contains 33 cytochromes P450 (CYPs), many more than the 18 observed in Streptomyces coelicolor A3(2). Some of the likely metabolic functions are reported together with their genomic location and bioinformatic analysis. Seven entirely new CYP families were found together with close homologues of some forms observed in S. coelicolor A3(2). The presence of unusual CYP forms associated with conservons is revealed and of these, CYP157 forms in both S. avermitilis and S. coelicolor A3(2) deviate from the previously accepted rule for an EXXR motif within the K-helix of CYPs. Amongst this range of CYPs are forms associated with avermectin, filipin, geosmin, and pentalenolactone biosynthesis as well as unknown pathways of secondary metabolism. (C) 2003 Elsevier Inc. All rights reserved.