Single-Soma Deep RNA sequencing of Human DRG Neurons Reveals Novel Molecular and Cellular Mechanisms Underlying Somatosensation.
Single-Soma Deep RNA sequencing of Human DRG Neurons Reveals Novel Molecular and Cellular Mechanisms Underlying Somatosensation.
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人类 DRG 神经元的单体深度 RNA 测序揭示了体感的新分子和细胞机制。
DOI：
10.1101/2023.03.17.533207
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Wu
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作者：
Yu,Huasheng;Usoskin,Dmitry;Nagi,SaadS;Hu,Yizhou;Kupari,Jussi;Bouchatta,Otmane;Cranfill,SunaLi;Gautam,Mayank;Su,Yijing;Lu,You;Wymer,James;Glanz,Max;Albrecht,Phillip;Song,Hongjun;Ming,Guo-Li;Prouty,Stephen;Seykora,John;Wu
躯体感觉的多功能性来自于异质的人类背根神经节（DRG）神经元。由于技术上的困难，破译其功能的关键信息，即索马转录组，是缺乏的。在这里，我们开发了一种新的方法来分离单个人类DRG神经元胞体进行深度RNA测序（RNA-seq）。平均而言，每个神经元检测到> 9000个独特基因，并鉴定出16种神经元类型。跨物种分析显示，触摸，冷，痒感神经元类型相对保守，而疼痛感神经元显示出显着的分歧。人DRG神经元的索马转录组预测了新的功能特征，这是使用单细胞在体内电生理记录证实。这些结果支持了人类感觉传入神经的生理特性与单胞体RNA-seq数据集揭示的分子谱之间的密切关系。总之，通过对人类DRG神经元进行单胞体RNA-seq，我们为人类躯体感觉生成了前所未有的神经图谱。（未经同行评审认证）是作者/资助者。All rights reserved.未经许可不得重复使用。本预印本的版权保持器持有人于2023年3月18日发布。网址：//doi. org/10.1101/2023.03。17.533207 doi：bioRxiv预印本
The versatility of somatosensation arises from heterogenous human dorsal root ganglion (DRG) neurons. The critical information to decipher their functions, ie, the soma transcriptome, is lacking due to technical difficulties. Here we developed a novel approach to isolate individual human DRG neuron somas for deep RNA sequencing (RNA-seq). On average,> 9000 unique genes per neuron were detected, and 16 neuronal types were identified. Cross-species analyses revealed that touch-, cold-, and itch-sensing neuronal types were relatively conserved, while the pain-sensing neurons displayed marked divergence. Soma transcriptomes of human DRG neurons predicted novel functional features, which were confirmed using single-cell in vivo electrophysiological recordings. These results support a close relationship the between physiological properties of human sensory afferents and molecular profiles uncovered by the single-soma RNA-seq dataset. In summary, by conducting single-soma RNA-seq of human DRG neurons, we generated an unprecedented neural atlas for human somatosensation.(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. The copyright holder for this preprint this version posted March 18, 2023.; https://doi. org/10.1101/2023.03. 17.533207 doi: bioRxiv preprint