SIMULTANEOUS MEASUREMENT OF BLOOD-FLOW AND GLUCOSE-METABOLISM BY AUTORADIOGRAPHIC TECHNIQUES
SIMULTANEOUS MEASUREMENT OF BLOOD-FLOW AND GLUCOSE-METABOLISM BY AUTORADIOGRAPHIC TECHNIQUES
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DOI：
10.1161/01.str.12.5.581
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发表时间：
1981-01-01
期刊：
影响因子：
8.3
通讯作者：
HOSSMANN, KA
中科院分区：
文献类型：
--
作者：
MIES, G;NIEBUHR, I;HOSSMANN, KA
本文介绍了一种用131 I-碘-安替比林和14 C-脱氧葡萄糖的双示踪剂放射自显影技术，用于同时测量同一动物[大鼠]的血流量和脑葡萄糖利用率。131 I是γ。放射性同位素的半衰期为8.06天，并且可以在标准放射自显影胶片上以足够的分辨率检测。在131 I衰变前后进行放射自显影;调整两次曝光之间的时间间隔和两种示踪剂的浓度，以避免明显的交叉污染。以这种方式，获得2个胶片曝光，其可以像单个示踪剂放射自显影图一样定量处理。在各种生理和病理生理条件下的流量和代谢的局部耦合的调查方法的有效性进行了测试。在巴比妥酸盐麻醉的健康动物中，偶联是紧密的，但在氟烷麻醉下，在各种皮质下结构中发生解偶联。局部应用青霉素诱发的局灶性癫痫发作导致丘脑中继核团的代谢和血流增加，但青霉素给药部位的代谢和血流增加并不伴随类似的血流增加。在扩散性抑郁和实验性脑肿瘤的限定区域中观察到局部葡萄糖利用的偶联和非偶联增加。双示踪剂放射自显影允许非常精确的局部评估脑血流量和葡萄糖的利用，特别适合于在各种实验条件下的区域耦合过程的研究。
A double tracer autoradiographic technique using 131I-iodo-antipyrine and 14C-deoxyglucose is presented for the simultaneous measurement of blood flow and cerebral glucose utilization in the same animal [rat]. 131I is a .gamma.-emitting isotope with a half life of 8.06 days and can be detected with adequate resolution on standard autoradiographic films. Autoradiograms are made before and after decay of 131I; the time interval between the 2 exposures and the concentration of the 2 tracers is adjusted to avoid significant cross-contamination. In this way, 2 film exposures are obtained which can be processed quantitatively like single tracer autoradiograms. The validity of the method for the investigation of local coupling of flow and metabolism was tested under various physiological and pathophysiological conditions. Coupling was tight in barbiturate-anesthetized healthy animals, but not under halothane anesthesia where uncoupling occurred in various subcortical structures. Focal seizures incuded by topical application of penicillin on the cortical surface led to a coupled increase of metabolism and flow in thalamic relay nuclei but not at the site of penicillin administration where increased glucose utilization was not accompanied by similar increased in blood flow. Both coupled and uncoupled increases in local glucose utilization were observed in spreading depression and in circumscribed areas of experimental brain tumors. Double tracer autoradiography allows the very precise local assessment of cerebral blood flow and glucose utilization, and is particularly suited to the study of regional coupling processes under various experimental conditions.