Sweet sorghum syrup as a renewable material for microbial lipid production
Sweet sorghum syrup as a renewable material for microbial lipid production
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DOI：
10.1016/j.bej.2014.09.013
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发表时间：
2015-01-15
影响因子：
3.9
通讯作者：
Liang, Yanna
中科院分区：
文献类型：
--
作者：
Cui, Yi;Liang, Yanna
弯曲隐球酵母是一株产油酵母菌，能在甜高粱糖浆上生长。在分批培养模式下，在总糖浓度为11.7 ~ 34.0 g/L的试验范围内，未检测到底物对细胞生长的抑制作用。C. curvatus依次利用葡萄糖、果糖和蔗糖。然而，在分批补料过程中，这种酵母更迅速地同时消耗三种糖。在三天内，获得了23.6g/L的生物质密度。总体脂质生产率为4.0 g/L-天。采用微波辅助提取法，以甲醇为唯一溶剂，4 min内可将78.1%的有效脂质从弯曲藻细胞中提取出来。本研究表明，C.使用高粱糖浆将导致高脂质产量。由爱思唯尔公司出版
Cryptococcus curvatus, an oleaginous yeast strain was observed to grow on sweet sorghum syrup derived from sorghum juice. In a batch cultivation mode, within the tested range of total sugar concentrations from 11.7 to 34.0 g/L, no substrate inhibition to cell growth was detected. C. curvatus utilized glucose, fructose, and sucrose sequentially. In a fed-batch process, however, this yeast consumed the three sugars more rapidly and simultaneously. Within three days, a biomass density of 23.6 g/L was obtained. The overall lipid productivity was 4.0 g/L-day. Through use of microwave-assisted lipid extraction, 78.1% of available lipid was extracted out of C curvatus cells in 4min using methanol as the only solvent. This study demonstrated that fermentation of C. curvatus using sorghum syrup will result in high lipid yield. Published by Elsevier B.V.