Green light irradiation during sex differentiation induces female-to-male sex reversal in the medaka Oryzias latipes
Green light irradiation during sex differentiation induces female-to-male sex reversal in the medaka Oryzias latipes
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DOI：
10.1038/s41598-019-38908-w
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发表时间：
2019-02-20
影响因子：
4.6
通讯作者：
Kotani, Tomonari
中科院分区：
文献类型：
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作者：
Hayasaka, Oki;Takeuchi, Yutaka;Kotani, Tomonari
本研究探讨了特定光波长的照射是否会影响鱼类的性别分化。我们首先发现，在青鳉性别分化期间，负责接收红色、绿色和紫外线的感光基因在青鳉的眼睛中表达。其次，我们发现，睾丸发育的基因型女性的15.9%，饲养在绿色光照射。这些雌性转雄性的鱼（即新雄性）表现出雄性特有的第二性征，并产生活动精子。最后，使用新雄性（XX）的精子和正常雌性（XX）的卵子进行的后代测试显示，所有F1后代都是雌性，这首次表明在动物中用特定波长的光照射可以引发性逆转。
This study investigated whether irradiation of a specific light wavelength could affect the sex differentiation of fish. We first found that the photoreceptor genes responsible for receiving red, green, and ultraviolet light were expressed in the eyes of medaka during the sex differentiation period. Second, we revealed that testes developed in 15.9% of genotypic females reared under green light irradiation. These female-to-male sex-reversed fish (i.e. neo-males) showed male-specific secondary sexual characteristics and produced motile sperm. Finally, progeny tests using the sperm of neo-males (XX) and eggs of normal females (XX) revealed that all F1 offspring were female, indicating for the first time in animals that irradiation with light of a specific wavelength can trigger sex reversal.