Laser irradiated fluorescent perfluorocarbon microparticles in 2-D and 3-D breast cancer cell models.
Laser irradiated fluorescent perfluorocarbon microparticles in 2-D and 3-D breast cancer cell models.
复制标题
2-D 和 3-D 乳腺癌细胞模型中的激光照射荧光全氟化碳微粒
DOI：
10.1038/srep43408
复制
发表时间：
2017-03-06
影响因子：
4.6
通讯作者：
Peng Q
中科院分区：
文献类型：
--
作者：
Niu C;Wang L;Wang Z;Xu Y;Hu Y;Peng Q
全氟化碳（PFC）微滴作为新一代超声造影剂，通过声学或光学微滴汽化（ADV或ODV）进行了研究。关于PFC-微粒复合物的ODV辐照蒸发机制和产生的新气泡的稳定性知之甚少。在这项研究中，荧光全氟己烷（PFH）的聚（乳酸-羟基乙酸）（PLGA）颗粒被用作模型，以研究在二维（2-D）和三维（3-D）细胞模型中的颗粒蒸发和气泡稳定性激发后的过程。我们在荧光显微镜下观察了最初和蒸发后荧光剂在微粒涂层材料上的定位。此外，蒸发后观察新产生的气泡的稳定性和生长动力学11分钟。该颗粒与二维细胞共培养形成三维球体，即使通过激光照射封装在球体内也可以蒸发，这为进一步的工作提供了有效的基础。
Perfluorocarbon (PFC) droplets were studied as new generation ultrasound contrast agents via acoustic or optical droplet vaporization (ADV or ODV). Little is known about the ODV irradiated vaporization mechanisms of PFC-microparticle complexs and the stability of the new bubbles produced. In this study, fluorescent perfluorohexane (PFH) poly(lactic-co-glycolic acid) (PLGA) particles were used as a model to study the process of particle vaporization and bubble stability following excitation in two-dimensional (2-D) and three-dimensional (3-D) cell models. We observed localization of the fluorescent agent on the microparticle coating material initially and after vaporization under fluorescence microscopy. Furthermore, the stability and growth dynamics of the newly created bubbles were observed for 11 min following vaporization. The particles were co-cultured with 2-D cells to form 3-D spheroids and could be vaporized even when encapsulated within the spheroids via laser irradiation, which provides an effective basis for further work.