Gene expression profile of aging in human muscle
Gene expression profile of aging in human muscle
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DOI：
10.1152/physiolgenomics.00049.2003
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发表时间：
2003-07-07
影响因子：
4.6
通讯作者：
Thornton, CA
中科院分区：
文献类型：
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作者：
Welle, S;Brooks, AI;Thornton, CA
与骨骼肌衰老相关的基因表达的研究包括很少的主题或有限数量的基因。我们进行本研究是为了产生更全面的基因表达谱。从健康年轻人（21 - 27岁，n = 8）和老年人（67 - 75岁，n = 8）的股外侧肌活检中提取RNA，并用高密度寡核苷酸阵列进行分析。在阵列上的44,000个探针组中，大约18,000个产生了足够的信号用于统计分析。有相似的700个探针集，其中t检验或秩和检验表明年轻人和老年人之间的平均表达存在差异（P小于或等于0.01），估计的错误发现率为
Studies of gene expression related to aging of skeletal muscle have included few subjects or a limited number of genes. We conducted the present study to produce more comprehensive gene expression profiles. RNA was extracted from vastus lateralis biopsies obtained from healthy young (21 - 27 yr old, n = 8) and older men (67 - 75 yr old, n = 8) and was analyzed with high-density oligonucleotide arrays. Of the similar to44,000 probe sets on the arrays, similar to18,000 yielded adequate signals for statistical analysis. There were similar to700 probe sets for which t-tests or rank sum tests indicated a difference (P less than or equal to 0.01) in mean expression between young and old and for which the estimated false discovery rate was