Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip.
Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip.
复制标题
通过排列在凹坑芯片中的模型不对称膜军研究蛋白质运输。
DOI：
10.1007/978-1-0716-0806-7_16
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Chen,Min
中科院分区：
文献类型：
--
作者：
Li,Xin;Chen,Min
重建的模型膜系统是解决膜生物学中存在的有趣问题的强大平台。作为疾病治疗方法的有效药物递送的障碍之一是细胞的物理膜屏障。小分子通常可以通过膜扩散；然而，诸如蛋白质或核酸之类的生物分子不能被动地扩散双层，因此许多研究都致力于设计蛋白质和/或核酸递送方法。一种递送方法使用细胞穿透肽（CPP）。在本章中，我们介绍了排列在凹坑芯片中的模型“膜军”，以研究称为 Pep-1 的 CPP 传递 β-半乳糖苷酶。该方法使用液滴界面双层技术（DIB）。它加快了筛选 CPP 辅助蛋白质易位工作条件的速度，因为每个芯片都提供可容纳 36 对液滴或 18 个模型双层的凹坑。我们将以β-半乳糖苷酶递送的成功易位条件之一为例，说明如何构建和利用“膜军队”模型。
Reconstituted model membrane systems are powerful platforms to tackle interesting problems existing in membrane biology. One of the barriers to efficient drug delivery, as therapeutics to disease, is the physical membrane barrier of the cell. Small molecule can typically diffuse through the membrane; however, biomolecules such as proteins or nucleic acids cannot passively diffuse the bilayer and thus much research has been geared to engineering protein and/or nucleic acids delivery methods. One delivery method uses cell penetrating peptides (CPPs). In this chapter, we introduce the model “membrane army” arranged in dimple chip to study the delivery of β-galactosidase by a CPP known as Pep-1. This method uses droplet interface bilayer technology (DIB). It accelerates the speed to screen through the working conditions in CPP-assisted protein translocations because each chip provides dimples that can accommodate 36 pairs of droplets or 18 model bilayers. We will use one of the successful translocation conditions of β-galactosidase delivery as the example to illustrate how the model “membrane army” is built and utilized.